De novo transcriptome assembly and identification of salt-responsive genes in sugar beet M14
De novo transcriptome assembly and identification of salt-responsive genes in sugar beet M14
复制标题
甜菜 M14 中盐响应基因的从头转录组组装和鉴定
DOI：
10.1016/j.compbiolchem.2018.04.014
复制
发表时间：
2018-08-01
影响因子：
3.1
通讯作者：
Wang, Yuguang
中科院分区：
文献类型：
--
作者：
Lv, Xiaoyan;Jin, Ying;Wang, Yuguang
甜菜是世界上重要的制糖作物。已有研究表明，栽培甜菜与野生甜菜杂交获得的甜菜单体附加系M14具有较强的耐盐性。估计与M14耐盐性有关的广泛基因将有助于阐明盐胁迫的分子机制。以甜菜M14幼苗为材料，采用比较转录组学方法，对0、200和400 mM盐胁迫下甜菜M14幼苗叶片和根部差异表达基因进行了检测。数字基因表达结果表明，盐胁迫下，叶片中有3856个Uniges差异表达，根中有7157个Uniges差异表达。基于GO和KEGG数据库的差异表达基因分析表明，在叶片和根中，与氧化还原平衡调节、信号转导和蛋白磷酸化相关的基因都有差异表达。比较了200 mM和400 mM盐处理的叶片和根的基因表达，揭示了不同的盐胁迫处理机制。此外，在活性氧清除系统中，9种Uniges在叶片和根中的表达水平存在显著差异。我们的转录组学结果为甜菜叶片和根对盐胁迫的反应提供了新的见解。(C)2018爱思唯尔有限公司。保留所有权利。
Sugar beet (Beta vulgaris) is an important crop of sugar production in the world. Previous studies reported that sugar beet monosomic addition line M14 obtained from the intercross between Beta vulgaris L. (cultivated species) and B. corolliflora Zoss (wild species) exhibited tolerance to salt (up to 0.5 M NaCl) stress. To estimate a broad spectrum of genes involved in the M14 salt tolerance will help elucidate the molecular mechanisms underlying salt stress. Comparative transcriptomics was performed to monitor genes differentially expressed in the leaf and root samples of the sugar beet M14 seedlings treated with 0, 200 and 400 mM NaCl, respectively. Digital gene expression revealed that 3856 unigenes in leaves and 7157 unigenes in roots were differentially expressed under salt stress. Enrichment analysis of the differentially expressed genes based on GO and KEGG databases showed that in both leaves and roots genes related to regulation of redox balance, signal transduction, and protein phosphorylation were differentially expressed. Comparison of gene expression in the leaf and root samples treated with 200 and 400 mM NaCl revealed different mechanisms for coping with salt stress. In addition, the expression levels of nine unigenes in the reactive oxygen species (ROS) scavenging system exhibited significant differences in the leaves and roots. Our transcriptomics results have provided new insights into the salt-stress responses in the leaves and roots of sugar beet. (C) 2018 Elsevier Ltd. All rights reserved.