Kangen-karyu raises surface body temperature through oxidative stress modification.
Kangen-karyu raises surface body temperature through oxidative stress modification.
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DOI：
10.3164/jcbn.15-135
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发表时间：
2016-05
影响因子：
2.4
通讯作者：
Aoyagi K
中科院分区：
文献类型：
--
作者：
Hirayama A;Okamoto T;Kimura S;Nagano Y;Matsui H;Tomita T;Oowada S;Aoyagi K
Kangen-karyu是一种含有六种草药的处方，已被证明通过动物模型中的氧化应激依赖性途径实现其药理作用。本研究的目的是研究还原剂的抗氧化作用与药理学机制之间的关系，特别是其对人体的体温升高作用。本研究招募了年龄为35 ± 15岁的健康人类志愿者。体表体温，血清亚硝酸盐，活性氧（ROS）清除活性，和炎性细胞因子进行了研究之前和120分钟后Kangen-karyu口服摄入。还原karyu显着提高了全身的体表温度;这种效果在上身更显着，并持续超过120分钟。伴随着这种治疗效果，口服给药后120分钟血清亚硝酸盐水平升高。血清活性氧清除活性增强对单线态氧，同时对烷氧基自由基下降。血清亚硝酸盐水平和超氧阴离子清除活性呈正相关，表明还原核汤影响体内O2·−-NO平衡。还原剂对IL-6、TNF-α和脂联素水平无影响。这些结果表明，还原剂的治疗效果是通过NO和ROS依赖性机制实现的。此外，该机制不限于ROS产生，而是包括ROS-ROS或ROS-NO相互作用。
Kangen-karyu, a prescription containing six herbs, has been shown to achieve its pharmacological effect through oxidative stress-dependent pathways in animal models. The aim of this study is to investigate the relationship between the antioxidative effect and pharmacological mechanisms of Kangen-karyu, specifically its body temperature elevating effect in humans. Healthy human volunteers, age 35 ± 15 years old, were enrolled in this study. Surface body temperature, serum nitrite, reactive oxygen species (ROS) scavenging activities, and inflammatory cytokines were investigated before and 120 min after Kangen-karyu oral intake. Kangen-karyu significantly increased the surface-body temperature of the entire body; this effect was more remarkable in the upper body and continued for more than 120 min. Accompanying this therapeutic effect, serum nitrite levels were increased 120 min after oral administration. Serum ROS scavenging activities were enhanced against singlet oxygen and were concomitantly decreased against the alkoxyl radical. Serum nitrite levels and superoxide scavenging activities were positively correlated, suggesting that Kangen-karyu affects the O2•−-NO balance in vivo. Kangen-karyu had no effect on IL-6, TNF-α and adiponectin levels. These results indicate that the therapeutic effect of Kangen-karyu is achieved through NO- and ROS-dependent mechanisms. Further, this mechanism is not limited to ROS production, but includes ROS-ROS or ROS-NO interactions.