STUDIES ON THE STRUCTURE OF THE VITREOUS BODY .12. CYTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE CORTICAL TISSUE LAYER
STUDIES ON THE STRUCTURE OF THE VITREOUS BODY .12. CYTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE CORTICAL TISSUE LAYER
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DOI：
10.1016/s0014-4835(64)80008-7
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发表时间：
1964-01-01
影响因子：
3.4
通讯作者：
MITCHELL, AP
中科院分区：
文献类型：
--
作者：
BALAZS, EA;TOTH, LZJ;MITCHELL, AP
在成年哺乳动物和鸟类中，玻璃体的皮质组织层是一种固体组织，与睫状体和视网膜相邻，其中包含一种称为“透明细胞”的均匀细胞群。只有与视盘相邻的区域含有另一种结缔组织细胞，即纤维细胞。玻璃体每表面积细胞数量最多的是睫状体和视盘。透明质细胞的颗粒在活态和固定后表现出对光和氧敏感的自身荧光，并吸收阳离子染料。颗粒中的pas阳性嗜碱性物质不能通过透明质酸酶或神经氨酸酶处理去除。组胺解放剂b.w.48 -80对颗粒无影响。透明细胞的动态装置在电影显微摄影中通过细胞膜、颗粒、囊泡和线粒体的运动以及整个细胞的变形虫运动来证明。表面囊泡的形成是细胞两种截然不同的退行过程之一的特征。用中性红色对颗粒进行重要染色可启动光动力学过程，从而导致颗粒中染料的减少和细胞退化的加速。动物死后，皮层组织层表面迅速形成嗜碱性液滴层。这些液滴被解释为来自视网膜和玻璃体的大分子的聚集。
The cortical tissue layer of the vitreous body, in adult mammals and in birds, is a solid tissue which is contiguous to the ciliary body and the retina and which contains a uniform population of cells called "hyalocytes". Only the area contiguous to the optic disc contains another type of connective tissue cell, viz. fibrocytes. The largest number of cells per surface area of vitreous body is found at the ciliary body and at the optic disk. The granules of the hyalocytes show light- and oxygen-sensitive autofluorescence and take up cationic dyes in the living state and after fixation. The PAS-positive basophilic material in the granules cannot be removed by treatment with hyaluronidase or neuraminidase. Histamine liberator B. W. 48-80 does not affect the granules. The dynamic apparatus of the hyalocytes is demonstrated in cinephotomicrography by the movements of the cell membranes, granules, vesicles and mitochondria, as well as by the amoeboid movement of the whole cell. The formation of surface vesicles characterizes one of the two distinctly different degenerative processes of the cells. Vital staining of the granules with neutral red initiates a photodynamic process, which results in a reduction of the dye in the granules and an acceleration of the cell degeneration. A basophilic droplet layer rapidly develops on the surface of the cortical tissue layer after the death of the animal. The droplets are interpreted as a coacervation of macromolecules originating from the retina and the vitreous body.