Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans
Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans
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DOI：
10.1038/s42003-019-0407-5
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发表时间：
2019-05-02
影响因子：
5.9
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
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作者：
Matsumoto, Yasuhiko;Ishii, Masaki;Sekimizu, Kazuhisa
过量摄入蔗糖引起的高血压会导致与生活方式有关的疾病，如糖尿病。向小鼠施用乳酸菌菌株可以抑制蔗糖诱导的高血糖症，但缺乏在人类中产生类似效果的证据。在这里，我们表明，粪肠球菌YM 0831，确定使用体内筛选系统与蚕，抑制蔗糖诱导的高血糖症在人类。E. faecalis YM 0831还抑制蚕中葡萄糖诱导的高血糖症。E. faecalis YM 0831抑制人肠上皮细胞系Caco-2的葡萄糖摄取。一个E. faecalis YM 0831对Caco-2细胞的葡萄糖摄取显示出降低的抑制活性，其对蚕中蔗糖诱导的和葡萄糖诱导的高血糖症也显示出降低的抑制活性。在人体临床试验中，口服E. faecalis YM 0831在蔗糖耐受性试验中抑制血糖的增加。这些结果表明，E. faecalis YM 0831抑制肠道葡萄糖转运并抑制蔗糖诱导的人体高血糖症。
Hyperglycemia caused by excessive intake of sucrose leads to lifestyle-related diseases such as diabetes. Administration of a lactic acid bacterial strain to mice suppresses sucrose-induced hyperglycemia, but evidence for a similar effect in humans is lacking. Here we show that Enterococcus faecalis YM0831, identified using an in vivo screening system with silk-worms, suppressed sucrose-induced hyperglycemia in humans. E. faecalis YM0831 also suppressed glucose-induced hyperglycemia in silkworms. E. faecalis YM0831 inhibited glucose uptake by the human intestinal epithelial cell line Caco-2. A transposon insertion mutant of E. faecalis YM0831, which showed decreased inhibitory activity against glucose uptake by Caco-2 cells, also exhibited decreased inhibitory activity against both sucrose-induced and glucose-induced hyperglycemia in silkworms. In human clinical trials, oral ingestion of E. faecalis YM0831 suppressed the increase in blood glucose in a sucrose tolerance test. These findings suggest that E. faecalis YM0831 inhibits intestinal glucose transport and suppresses sucrose-induced hyperglycemia in humans.