Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in cultured tobacco cells
Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in cultured tobacco cells
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DOI：
10.1093/pcp/pch105
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Moriyasu, Y
中科院分区：
文献类型：
--
作者：
Yano, K;Matsui, S;Moriyasu, Y
在半胱氨酸蛋白酶抑制剂存在下，在蔗糖饥饿条件下培养的烟草细胞中，自溶酶体积累。我们使用荧光染料和绿色荧光蛋白（GFP）对这些植物自溶体进行了表征。使用内吞标记物FM 4 -64和荧光黄CH的观察表明，存在从质膜到自溶酶体的膜流。使用这些染料以及GFP-AtVam 3 p，sporamin-GFP和gamma-VM 23-GFP融合蛋白作为中央液泡的标记，我们发现中央液泡的组分运输到自溶酶体。因此，内吞作用和中央液泡的供应可能有助于自溶酶体的形成。
Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent dyes and green fluorescent protein (GFP). Observation using the endocytosis markers, FM4-64 and Lucifer Yellow CH, suggested that there is a membrane flow from the plasma membrane to autolysosomes. Using these dyes as well as GFP-AtVam3p, sporamin-GFP and gamma-VM23-GFP fusion proteins as markers of the central vacuole, we found transport of components of the central vacuole to autolysosomes. Thus endocytosis and the supply from the central vacuole may contribute to the formation of autolysosomes.