CLONING AND CHARACTERIZATION OF A HUMAN ANGIOTENSIN-II TYPE-1 RECEPTOR
CLONING AND CHARACTERIZATION OF A HUMAN ANGIOTENSIN-II TYPE-1 RECEPTOR
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DOI：
10.1016/s0006-291x(05)80288-8
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发表时间：
1992-03-31
影响因子：
3.1
通讯作者：
AIYAR, N
中科院分区：
文献类型：
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作者：
BERGSMA, DJ;ELLIS, C;AIYAR, N
以大鼠1型血管紧张素II受体cDNA编码序列为探针，筛选人肝cDNA文库。分离cDNA克隆，其编码359个氨基酸的蛋白质，分别与大鼠和牛1型血管紧张素II受体具有94.4%和95.3%的同一性。配体结合实验表明，该受体是一种1型血管紧张素Ⅱ受体亚型。对非洲爪蟾细胞中表达的受体的电生理研究表明，它可以在功能上与第二信使系统偶联，导致细胞内钙储存的动员。Southern和北方印迹分析表明，克隆的受体在人类基因组中表现为单拷贝，并在不同组织起源的许多组织中表达，但脑除外，其中mRNA转录物几乎检测不到。
A human liver cDNA library was screened using a rat type 1 angiotensin II receptor cDNA coding sequence as a probe. cDNA clones were isolated which encoded a protein of 359 amino acids that shared 94.4% and 95.3% identity to rat and bovine type 1 angiotensin II receptors, respectively. Ligand binding studies of the cloned receptor expressed in COS cells suggested that it is pharmacologically a type 1 angiotensin II receptor subtype. Electrophysiological studies of the receptor expressed inXenopus laevisoocytes revealed that it could functionally couple to a second messenger system leading to the mobilization of intracellular stores of calcium. Southern and Northern blot analyses indicated that the cloned receptor is represented as a single copy in the human genome and is expressed in many tissues of different histogenic origin with the exception of brain, where mRNA transcripts were barely detectable.