Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis
Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis
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DOI：
10.1089/pho.2011.3080
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Ochiai, Naoyuki
中科院分区：
文献类型：
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作者：
Funayama, Toru;Sakane, Masataka;Ochiai, Naoyuki
目的：本研究旨在探讨吲哚菁绿色（ICG）光动力疗法（PDT）联合近红外光照射对大鼠乳腺癌细胞的光毒性作用及其对大鼠脊柱转移瘤的治疗效果。背景资料：尽管PDT已成功地用于许多恶性肿瘤的非放射治疗，但其尚未临床应用于治疗脊柱转移瘤。方法：采用PDT处理CRL-1666细胞，WST-1法检测细胞活力。对于有效性研究，将26只在L 6椎骨中具有脊柱转移的雌性Fischer 344大鼠分为三个治疗组：局部注射ICG的PDT（9只大鼠）、全身注射ICG的PDT（10只大鼠）和无治疗或对照（7只大鼠）。两组均接受总能量为10 J的近红外光照射（1 W，10 sec）。光直接通过设置在L 6椎体左侧的单个二氧化硅探针传输。根据Basso-Beattie-Bresnahan（BBB）量表监测后肢运动功能。此外，计算观察期以确定存活时间。结果：PDT具有即刻和持续的光毒性作用。此外，局部注射ICG以及全身注射ICG的PDT延缓了瘫痪的恶化，延长了观察期。结论：PDT联合ICG注射和近红外光照射是治疗脊柱转移瘤的有效局部辅助治疗方法。
Objective: The purpose of this study was to investigate the phototoxic effects of photodynamic therapy (PDT) with indocyanine green (ICG) and near-infrared light irradiation on rat mammary adenocarcinoma cells, and its therapeutic efficacy in a rat model of spinal metastasis. Background data: Although PDT has been successfully used as a non-radiation treatment for many malignancies, it has not yet been clinically applied for treating spinal metastasis. Methods: For the phototoxicity study, CRL-1666 cells were treated with PDT and cell viability was measured by WST-1 assay. For the efficacy study, 26 female Fischer 344 rats with spinal metastasis in the L6 vertebra were divided into three treatment groups: PDT with local injection of ICG (9 rats), PDT with systemic injection of ICG (10 rats), and no treatment or control (7 rats). Both the PDT groups received near-infrared light irradiation with a total energy of 10 J (1W for 10 sec). The light was delivered directly through a single silica probe which was set on the left side of the L6 vertebral body. Hindlimb motor function was monitored according to the Basso-Beattie-Bresnahan (BBB) scale. Further, the observation periods were calculated to determine the survival time. Results: The PDT exerted immediate and persistent phototoxic effects. Furthermore, the PDT with local injection of ICG as well as systemic injection of ICG delayed the deterioration of paralysis and prolonged the observation period. Conclusions: PDT with ICG injection and near-infrared light irradiation could be an effective local adjuvant treatment for spinal metastasis.