Efferent vascular patterns and early vascular-tubular relations in the dog kidney.
Efferent vascular patterns and early vascular-tubular relations in the dog kidney.
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狗肾脏的传出血管模式和早期血管-小管关系。
DOI：
10.1152/ajplegacy.1971.221.5.1361
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
R. Beeuwkes
中科院分区：
文献类型：
--
作者：
R. Beeuwkes
方法输精管。对犬肾注射硅橡胶后肾小球血管通路进行了研究(2)。用快速静脉注射过量戊巴比妥钠处死杂种犬，雌雄不拘。通过侧腹或腹部切开快速取出肾脏，并用适当大小的聚乙烯管插管肾动脉。硅胶材料由等量的Microfi MV-112(科罗拉多州博尔德的广州生物医疗产品公司)组成。MV稀释剂，输液前加入体积分数为3%的二月桂酸二丁基锡催化剂。将血管插管连接到含有橡胶化合物的恒压容器上，并将输液保持在75或150 mm Hg的压力下至少4分钟。然后夹住肾蒂，在室温下用夹子将器官悬吊起来，直到橡胶固化，通常不到一小时。然后将肾脏冷藏一夜，并将其切成垂直于其长轴的切片。这些切片在增加酒精浓度的情况下脱水，通常在每个浓度下保持24小时。将它们从无水乙醇中转移到水杨酸甲酯(冬青油)中进行清除和储存。血管与小管的关系。通过活体固定肾脏，然后动脉注射硅胶材料来证明血管与肾小管的关系。然后对肾脏进行脱水、清洗和切片。在直接观察下，将微管插入皮质各部位肾小球的尿腔，并经肾小球注入颜色对比的硅橡胶近端小管。
METHODSEferent vessels. The postglomerular vascular pathways were studied in dog kidneys injected with silicone rubber (2). Mongrel dogs of both sexes were sacrificed by rapid intravenous overdose of pentobarbital. The kidneys were rapidly removed through a flank or abdominal incision and the renal artery was cannulated with polyethylene tubing of appropriate size. The silicone material consisted of equal parts of Microfil MV-112 (Canton Biomedical Products, Boulder, Colo.) and MV diluent to which 3% by volume of dibutyltin dilaurate catalyst was added just before infusion. The vascular cannula was connected to a constant-pressure reservoir containing the rubber compound and the infusion maintained at a pressure of 75 or 150 mm Hg for at least 4 min. The renal pedicle was then clamped and the organ was suspended by this clamp at room temperature until the rubber had cured, usually less than an hour. The kidney was then refrigerated overnight and cut into slices perpendicular to its long axis. These slices were dehydrated in increasing concentrations of alcohol, usually remaining in each concentration for 24 hr. They were transferred from absolute alcohol to methyl salicylate (oil of wintergreen) for clearing and storage.Vascular-tubular relations. Vascular-tubular relations were demonstrated by in vivo fixation of the kidney, followed by arterial injection of silicone material. The kidneys were then dehydrated, cleared, and sliced. Under direct observation micropipets were maneuvered into the urinary spaces of glomeruli in each part of the cortex, and proximal tubules injected via the glomerulus with silicone rubber of contrasting color.