GELATINASE SECRETION BY GLOMERULAR EPITHELIAL-CELLS
GELATINASE SECRETION BY GLOMERULAR EPITHELIAL-CELLS
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DOI：
10.1159/000186184
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发表时间：
1990-12-01
期刊：
影响因子：
2.5
通讯作者：
NAKAGAWA, H
中科院分区：
文献类型：
--
作者：
WATANABE, K;KINOSHITA, S;NAKAGAWA, H
在海曼肾病大鼠的肾小球培养中观察到明胶溶解活性显著增加。培养基酶谱分析表明，主要明胶酶的分子量为98千道尔顿（kDa），具有金属蛋白酶的特征。在肾小球上皮细胞培养液中检测到98-kDa明胶酶，内皮细胞和系膜细胞培养液中未检测到。白介素- 1. - beta。，肿瘤坏死因子。脂多糖剂量依赖性地增加上皮细胞明胶酶的产生。提示细胞因子刺激下肾小球上皮细胞分泌的明胶酶参与了海曼肾炎的病理过程。
A significant increase in gelatinolytic activity was observed in cultures of glomeruli from Heymann nephritic rats. Zymography of the culture medium indicated that the main gelatinase species has a molecular weight of 98 kilodaltons (kDa) and the characteristics of metalloproteinase. The 98-kDa gelatinase was detected in the culture medium of glomerular epithelial cells but not in those of endothelial and mesangial cells. Interleukin-1.beta., tumor necrosis factor-.alpha. and lipopolysaccharide dose-dependently increased gelatinase production by epithelial cells. These results suggest that the gelatinase secreted by cytokine-stimulated glomerular epithelial cells participates in the pathological process of Heymann nephritis.