Complete DNA sequence and comparative analysis of the 50-kilobase virulence plasmid of Salmonella enterica serovar choleraesuis
Complete DNA sequence and comparative analysis of the 50-kilobase virulence plasmid of Salmonella enterica serovar choleraesuis
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DOI：
10.1128/iai.69.4.2612-2620.2001
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发表时间：
2001-04-01
影响因子：
3.1
通讯作者：
Danbara, H
中科院分区：
文献类型：
--
作者：
Haneda, T;Okada, N;Danbara, H
猪霍乱沙门氏菌RF-1株的大毒力质粒pKDSC 50的核苷酸全序列已被测定。我们鉴定了由49，503-bp分子编码的48个开放阅读框（ORF）。pKDSC 50编码一种已知的毒力相关操纵子，即间谍操纵子，它由非伤寒沙门氏菌全身感染所必需的基因组成。pKDSC 50的遗传结构分析表明，该质粒由几个毒力相关基因组成，其中包括sppRABCD基因、质粒复制和维持基因以及一个插入序列元件。第二个毒力相关区包括质粒编码的菌毛（pef）操纵子和补体杀伤抗性（Ick）基因，已在沙门氏菌毒力质粒上鉴定。肠伤寒血清型鼠伤寒不存在。结果表明，该质粒与肠出血性大肠杆菌O 157：H7的pO 157质粒和肠致病性大肠杆菌O 157：H7的pO 157质粒具有高度的相似性。EPEC B171（O 111：NM）携带的EPEC粘附因子质粒，以及鼠伤寒沙门氏菌和肠炎沙门氏菌的毒力质粒。对猪霍乱血清型50-kb毒力质粒和鼠伤寒血清型94-kb毒力质粒的核苷酸序列进行比较分析，发现猪霍乱血清型毒力质粒的48个ORF中有47个与鼠伤寒血清型毒力质粒的相应ORF高度同源，表明猪霍乱血清型和鼠伤寒血清型毒力质粒具有共同的祖先。
The complete nucleotide sequence of pKDSC50, a large virulence plasmid from Salmonella enterica serovar Choleraesuis strain RF-1, has been determined. We identified 48 of the open reading frames (ORFs) encoded by the 49,503-bp molecule. pKDSC50 encodes a known virulence associated operon, the spy operon, which is composed of genes essential for systemic infection by nontyphoidal Salmonella. Analysis of the genetic organization of pKDSC50 suggests that the plasmid is composed of several virulence-associated genes, which include the sppRABCD genes, plasmid replication and maintenance genes, and one insertion sequence element. A second virulence associated region including the pef (plasmid-encoded fimbria) operon and I ck (resistance to complement killing) gene, which has been identified on the virulence plasmid of S. enterica serovar Typhi murium, was absent. Two different replicon regions, similar to the RepFIIA and RepFIB replicons, were found. Both showed high similarity to those of the pO157 plasmid of enterohemorrhagic Escherichia coli O157:H7 and the enteropathogenic E. coli (EPEC) adherence factor plasmid harbored by EPEC strain B171 (O111:NM), as well as the virulence plasmids of Salmonella serovars Typhimurium and Enteritidis. Comparative analysis of the nucleotide sequences of the 50-kb virulence plasmid of serovar Choleraesuis and the 94-kb virulence plasmid of serovar Typhimurium revealed that 47 out of 48 ORFs of the virulence plasmid of serovar Choleraesuis are highly homologous to the corresponding ORFs of the virulence plasmid of serovar Typhimurium, suggesting a common ancestry.