An exported peptide functions intracellularly to contribute to cell density signaling in B-subtilis
An exported peptide functions intracellularly to contribute to cell density signaling in B-subtilis
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DOI：
10.1016/s0092-8674(00)80277-9
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发表时间：
1997-06-13
期刊：
影响因子：
64.5
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Lazazzera, BA;Solomon, JM;Grossman, AD
枯草芽孢杆菌的能力发育和孢子形成部分受到随着细胞生长至高密度而在培养基中积累的肽的控制。我们构建了两个基因，编码两种不同信号分子的成熟形式，即刺激孢子形成的PhrA肽和CSF（能力和孢子形成刺激因子）。两种五肽通常都是通过前体分子的分泌和加工产生的。成熟的五肽在细胞内表达时具有功能，这表明它们通常需要输入才能发挥作用。此外，在生理浓度 (10 nM) 下，CSF 通过 spoOK (opp) 编码的寡肽通透酶转运到细胞中。研究表明，CSF 具有与其三种活性相对应的至少三种不同靶标：低浓度下刺激能力基因表达，以及高浓度下抑制能力基因表达并刺激孢子形成。
Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and sporulation-stimulating factor. Both pentapeptides are normally produced by secretion and processing of precursor molecules. The mature pentapeptides were functional when expressed inside the cell, indicating that they normally need to be imported to function. Furthermore, at physiological concentrations (10 nM), CSF was transported into the cell by the oligopeptide permease encoded by spoOK (opp). CSF was shown to have at least three different targets corresponding to its three activities: stimulating competence gene expression at low concentrations, and inhibiting competence gene expression and stimulating sporulation at high concentrations.