Composite MR Contrast Agents for Conditional Cell-Labeling.
Composite MR Contrast Agents for Conditional Cell-Labeling.
复制标题
用于条件细胞标记的复合 MR 造影剂。
DOI：
10.1002/ima.20142
复制
发表时间：
2008
影响因子：
3.3
通讯作者：
Cho,Zang-Hee
中科院分区：
文献类型：
--
作者：
Marzelli,Matthew;Fischer,Krisztina;Kim,YoungBeom;Mulkern,RobertV;Yoo,Seung-Schik;Park,Hyunwook;Cho,Zang-Hee
钆螯合物（Gd-DTPA）和氧化铁超顺磁性颗粒（SPIO）是两种常用的MR造影剂，它们表现出固有的不同弛豫特性。这两种试剂已用于离体标记细胞，以在引入到感兴趣组织时产生相对于背景组织的信号对比。假设这两种药物之间的相互作用最小，我们的动机是通过将两种药物混合在水溶液中用于调节细胞标记来研究复合弛豫特性的产生。浓度依赖性弛豫系数首先从每种造影剂在3特斯拉的盐水溶液中独立获得。这些系数然后被用来预测的R1和R2弛豫率的复合造影剂使用的线性模型相结合的效果，两种造影剂。预测的弛豫速率通过25种复合溶液（SPIO-浓度范围为0至1 μg/mL，Gd-DTPA-浓度范围为0至0.20 mM的组合）进行了实验证实。我们表明，SPIO和Gd-DTPA在水溶液中的组合表现出独特且可预测的弛豫特性，这是单独使用任何一种试剂都无法实现的。该方法可应用于创建“用户可调”对比度条件，用于在细胞替代疗法的背景下可视化磁性标记的细胞。© 2008 Wiley Periodicals，Inc. Int J Imaging Syst Technol，18，79-84，2008
Gadolinium‐chelates (Gd‐DTPA) and superparamagnetic particles of iron oxide (SPIO) are two commonly used MR contrast agents that exhibit inherently different relaxation properties. These two agents have been used to label cells ex‐vivo to generate signal contrast with respect to background tissue when introduced to a tissue‐of‐interest. Assuming minimal mutual interaction between these two agents, we were motivated to investigate the creation of composite relaxation properties by mixing the two in aqueous solutions for conditioning cell labeling. Concentration‐dependent relaxivity coefficients were first obtained from each contrast agent, independently, in saline solution at 3 Tesla. These coefficients were then used to predict both theR1andR2relaxation rates of a composite contrast agent using a linear model combining the effects of both contrast media. The predicted relaxation rates were experimentally confirmed from 25 composite solutions (combinations of SPIO‐concentration ranging from 0 to 1 μg/mL and Gd‐DTPA‐concentration ranging from 0 to 0.20 mM). We show that the combination of SPIO and Gd‐DTPA in an aqueous solution exhibits unique and predictable relaxivity properties that are unattainable via the individual use of either agent. The method may be applied to create “user‐tunable” contrast conditions for the visualization of magnetically labeled cells in the context of cell replacement therapy. © 2008 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 18, 79–84, 2008