Differences in ASP1 expression and binding dynamics to queen mandibular pheromone HOB between Apis mellifera and Apis cerana workers reveal olfactory adaptation to colony organization.
Differences in ASP1 expression and binding dynamics to queen mandibular pheromone HOB between Apis mellifera and Apis cerana workers reveal olfactory adaptation to colony organization.
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DOI：
10.1016/j.ijbiomac.2022.07.064
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发表时间：
2022-07
影响因子：
8.2
通讯作者：
Fan Wu;Shenyun Liu;Xufeng Zhang;Han Hu;Qiaohong Wei;Bin Han;Hongliang Li
中科院分区：
文献类型：
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作者：
Fan Wu;Shenyun Liu;Xufeng Zhang;Han Hu;Qiaohong Wei;Bin Han;Hongliang Li
东方蜜蜂(Ac)和西方蜜蜂(Am)是两个关系密切、经济价值最高的蜜蜂物种，在全球范围内得到广泛管理。然而，工蜂是如何适应它们的蜂群组织的，还有待研究。在这里，我们发现在两种蜜蜂中，触角特异蛋白1(ASP1，识别皇后下颌信息素的关键调节因子)基因的表达水平与蜂群大小呈正相关，并且AM的表达水平高于INAC，这表明ASP1可能在维持蜂群动态平衡中发挥重要作用。通过竞争结合试验、分子对接和定点突变，我们证实了Ac-ASP1和Am-ASP1与对羟基苯甲酸甲酯(HOB)具有良好的结合亲和力，其中Val115是关键氨基酸。但Am-ASP1的亲和力强于Ac-ASP1。EAG的分析进一步证明，安工蜂的触角对HOB刺激有更快的去极化和复极化反应。综上所述，这些发现表明，表达水平和结合动力学的差异使ASP1识别HOB有可能成为AcandAM中菌落组织的特异性调节因子。
The easternApis cerana(Ac) and the westernApis mellifera(Am) are two closely related and most economically valuable honeybee species managed extensively worldwide. However, how worker bees ofAcandAmare adapted to their colony organization remains to be uncovered. Here, we found that the expression level of gene encoding antennae-specific proteins 1 (ASP1, a key regulator in recognizing queen mandibular pheromone) was positively correlated with the colony sizes in both bee species, and the expression level inAmwas higher than that inAc, suggesting that ASP1 may play an important role in maintaining colony homeostasis. Using competitive binding assay, molecular docking, and site-directed mutagenesis, we then confirmed the good binding affinities of both Ac-ASP1 and Am-ASP1 to methyl p-hydroxy benzoate (HOB), and Val115 was the key amino acid. However, the affinity of Am-ASP1 was stronger than that of Ac-ASP1. EAG analysis further demonstrated that antennae ofAmworker bees had faster depolarization and repolarization in response to HOB stimulation. Taken together, these findings indicate that the differences in expression levels and binding dynamics allow ASP1 recognizing HOB to potentially serve as a specific regulator of colony organization inAcandAm.