Amperometric Detection of Dopamine in Vivo with an Enzyme Based Carbon Fiber Microbiosensor
Amperometric Detection of Dopamine in Vivo with an Enzyme Based Carbon Fiber Microbiosensor
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DOI：
10.1021/ac9022605
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发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Andreescu, Silvana
中科院分区：
文献类型：
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作者：
Njagi, John;Chernov, Mykyta M.;Andreescu, Silvana
我们开发了一种新型的植入式酶基碳纤维生物传感器，用于在体监测多巴胺。该生物传感器是使用酪氨酸酶固定在生物相容性矩阵组成的生物聚合物，壳聚糖和二氧化铈基金属氧化物，沉积到碳纤维微电极的表面上的直径约为100 μ m。酪氨酸酶催化多巴胺转化为邻-多巴醌，并还原邻-多巴醌，这需要一个低的电位差，电化学检测。系统地研究了传感层中每个组分的作用与生物传感器的分析性能的关系。在其最佳配置中，生物传感器表现出1 nM多巴胺的检测限，在10 nM和220 μ M之间的5个数量级的线性范围，14.2 M-1的灵敏度，以及对抗坏血酸，尿酸，5-羟色胺，去甲肾上腺素，肾上腺素和3，4-二羟基-L-苯丙氨酸（L-DOPA）的良好选择性。nA.mu该系统提供了连续的，真实的时间监测电刺激多巴胺释放的麻醉大鼠的大脑。多巴胺水平高达1.69 μ M进行了测量。这种新的植入式多巴胺生物传感器提供了一种替代快速扫描循环伏安法在体内监测多巴胺。
We developed a novel implantable enzyme-based carbon fiber biosensor for in vivo monitoring of dopamine. The biosensor is fabricated using tyrosinase immobilized in a biocompatible matrix consisting of a biopolymer, chitosan and ceria-based metal oxides, deposited onto the surface of a carbon fiber microelectrode with a diameter of similar to 100 mu m. Tyrosinase catalyzes the conversion of dopamine to o-dopaquinone, and the reduction of o-dopaquinone, which requires a low potential difference, was detected electrochemically. The role of each component in the sensing layer was systematically investigated in relation to the analytical performance of the biosensor. In its optimal configuration, the biosensor demonstrated a detection limit of 1 nM dopamine, a linear range of 5 orders of magnitude between 10 nM and 220 mu M, a sensitivity of 14.2 nA.mu M-1, and good selectivity against ascorbic acid, uric acid, serotonin, norepinephrine, epinephrine, and 3,4-dihydroxy-L-phenylalanine (L-DOPA). The system provided continuous, real time monitoring of electrically stimulated dopamine release in the brain of an anesthetized rat. Levels of dopamine up to 1.69 mu M were measured. This new implantable dopamine biosensor provides an alternative to fast scan cyclic voltammetry for in vivo monitoring of dopamine.