The Tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy
The Tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy
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DOI：
10.1111/mpp.12144
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发表时间：
2014-12-01
影响因子：
4.9
通讯作者：
Kormelink, Richard
中科院分区：
文献类型：
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作者：
Hallwass, Mariana;De Oliveira, Athos Silva;Kormelink, Richard
虽然Sw-5基因簇已被克隆，并且Sw-5 b已被鉴定为番茄斑萎病毒（Tomato spotted wilt virus，TSWV）的功能基因拷贝，但其无毒（Avr）决定簇至今尚未被鉴定。用Sw-5 b基因的拷贝转化的烟草SR 1植物对TSWV攻击是免疫的，不产生明显的视觉反应，而这里显示用Sw-5 b转化的本塞姆氏烟草产生快速和明显的过敏反应（HR）。使用这些植物，从测试的所有结构和非结构TSWV蛋白中，使用马铃薯病毒X（PVX）复制子或非复制型pEAQ-HT表达载体系统确认TSWV细胞间移动蛋白（NSM）为Avr决定子。在转Sw-5 b基因的本氏烟以及抗性近等基因番茄品系中，在用来自抗性诱导（RI）TSWV株系（BR-01）的功能性细胞间运动蛋白（NSM）农杆菌渗入后诱导HR，但不使用来自Sw-5抗性破坏（RB）株系（GRAU）的NSM。这是第一次生物学证明Sw-5介导的抗性是由TSWV NSM细胞间运动蛋白触发的。
Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum SR1 plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N.benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N.benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU). This is the first biological demonstration that Sw-5-mediated resistance is triggered by the TSWV NSM cell-to-cell movement protein.