Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus.
Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus.
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DOI：
10.1111/j.1462-2920.2007.01285.x
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发表时间：
2007-07
影响因子：
5.1
通讯作者：
P. Weigele;W. Pope;M. Pedulla;Jennifer M. Houtz;Alexis L. Smith;J. Conway;J. King;G. Hatfull;J. Lawrence;R. Hendrix
中科院分区：
文献类型：
--
作者：
P. Weigele;W. Pope;M. Pedulla;Jennifer M. Houtz;Alexis L. Smith;J. Conway;J. King;G. Hatfull;J. Lawrence;R. Hendrix
蓝藻噬菌体Syn 9是一种大的，收缩尾的噬菌体，感染广泛分布的，数量上占优势的原绿球藻属和聚球藻属的海洋蓝藻。其177，300 bp的基因组序列编码226种推定蛋白质和6种tRNA。实验和计算分析确定了可能参与病毒体形成，核苷酸合成和DNA复制和修复的基因。当与相关的噬藻体S-PM2、P-SSM 2和P-SSM 4相比时，Syn 9显示出显著的镶嵌性，尽管共享的基因显示出强的纯化选择和相对于其他噬藻体的大种群规模的证据。与大肠杆菌噬菌体T4（共有Syn 9基因的19%）相关，Syn 9显示了不同DNA复制模式的证据，并使用同源蛋白组装具有不同整体结构的衣壳，与噬菌体SPO 1和疱疹病毒共享拓扑结构。Syn 9基因组中值得注意的细菌相关序列可能编码光合反应中心的亚基、电子传递蛋白、三种戊糖途径酶和两种色氨酸卤化酶。这些基因表明，Syn 9是很好地适应其光合宿主的生理，并可能会影响这些序列在海洋蓝藻的进化。
Cyanobacteriophage Syn9 is a large, contractile-tailed bacteriophage infecting the widespread, numerically dominant marine cyanobacteria of the genera Prochlorococcus and Synechococcus. Its 177,300 bp genome sequence encodes 226 putative proteins and six tRNAs. Experimental and computational analyses identified genes likely involved in virion formation, nucleotide synthesis, and DNA replication and repair. Syn9 shows significant mosaicism when compared with related cyanophages S-PM2, P-SSM2 and P-SSM4, although shared genes show strong purifying selection and evidence for large population sizes relative to other phages. Related to coliphage T4 - which shares 19% of Syn9's genes - Syn9 shows evidence for different patterns of DNA replication and uses homologous proteins to assemble capsids with a different overall structure that shares topology with phage SPO1 and herpes virus. Noteworthy bacteria-related sequences in the Syn9 genome potentially encode subunits of the photosynthetic reaction centre, electron transport proteins, three pentose pathway enzymes and two tryptophan halogenases. These genes suggest that Syn9 is well adapted to the physiology of its photosynthetic hosts and may affect the evolution of these sequences within marine cyanobacteria.