Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking
Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking
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DOI：
10.1063/1.2818767
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发表时间：
2007-10-01
期刊：
影响因子：
3.2
通讯作者：
Chang, Hsueh-Chia
中科院分区：
文献类型：
--
作者：
Gordon, Jason E.;Gagnon, Zachary;Chang, Hsueh-Chia
我们报告了一个有趣的缓冲液电弛豫时间调谐技术，再加上戊二醛交联细胞固定反应，它允许敏感的介电电泳分析和歧视牛红细胞（bRBC）饥饿年龄。选择缓冲液组成，使得存在两个容易获得的介电泳交叉频率（cof）。观察到低浓度戊二醛固定在较高的cof中产生三倍的降低，而在较低的cof中也观察到相当的增加。更重要的是，戊二醛固定浓度的增加显著增加了较高的cof，这是一个对bRBC饥饿年龄敏感的因素。(c)2007年，美国物理学会。
We report an interesting buffer electric relaxation time tuning technique, coupled with a glutaraldehyde cross-linking cell fixation reaction, which allows for sensitive dielectrophoretic analysis and discrimination of bovine red blood cell(bRBC) starvation age. The buffer composition is selected such that two easily accessible dielectrophoretic crossover frequencies (cof) exist. Low concentration glutaraldehyde fixation was observed to produce a threefold decrease in the higher cof with a comparable increase in the lower cof also witnessed. More importantly, increased glutaraldehyde fixation concentration significantly increased the higher cof by a factor found to be sensitive to the bRBC starvation age. (c) 2007 American Institute of Physics.