GENETIC-CONTROL OF MALE-FERTILITY IN ARABIDOPSIS-THALIANA - STRUCTURAL-ANALYSIS OF PREMEIOTIC DEVELOPMENTAL MUTANTS
GENETIC-CONTROL OF MALE-FERTILITY IN ARABIDOPSIS-THALIANA - STRUCTURAL-ANALYSIS OF PREMEIOTIC DEVELOPMENTAL MUTANTS
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DOI：
10.1007/bf00241884
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发表时间：
1994-01-01
影响因子：
--
通讯作者：
DENNIS, ES
中科院分区：
文献类型：
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作者：
CHAUDHURY, AM;LAVITHIS, M;DENNIS, ES
我们已经采取了突变的方法，以确定基因的雄性不育在拟南芥中的重要性，并已分离出一些核雄性/不育突变体，其中营养生长和女性生育力没有改变。在这里，我们描述了详细的发展分析的四个突变体，其中每一个定义了一个互补组，并有一个独特的发展终点。所有四个突变体代表减数分裂前发育病变。在ms 3中，绒毡层和中层肥大导致小孢子母细胞退化。在ms 4中，由于减数分裂受损，小孢子二分体在大部分花药发育中持续存在。在ms 5中，退化发生在发育早期的所有花药细胞中。在ms 15中，绒毡层和小孢子母细胞均在花药发育早期退化。这些突变体都有较短的花丝和更多的花序比同源雄性可育植物。这些突变体的发育表型的差异，以及突变的非等位基因性质表明，四个不同的基因对花粉发育很重要，已被确定。
We have taken a mutational approach to identify genes important for male fertility in Arabidopsis thaliana and have isolated a number of nuclear male/sterile mutants in which vegetative growth and female fertility are not altered. Here we describe detailed developmental analyses of four mutants, each of which defines a complementation group and has a distinct developmental end point. All four mutants represent premeiotic developmental lesions. In ms3, tapetum and middle layer hypertrophy result in the degeneration of microsporocytes. In ms4, microspore dyads persist for most of anther development as a result of impaired meiotic division. In ms5, degeneration occurs in all anther cells at an early stage of development. In ms15, both the tapetum and microsporocytes degenerate early in anther development. Each of these mutants had shorter filaments and a greater number of inflorescences than congenic male-fertile plants. The differences in the developmental phenotypes of these mutants, together with the non-allelic nature of the mutations indicate that four different genes important for pollen development, have been identified.