Hypolipidemic potential of squid homogenate irrespective of a relatively high content of cholesterol.
Hypolipidemic potential of squid homogenate irrespective of a relatively high content of cholesterol.
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DOI：
10.1186/1476-511x-13-165
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发表时间：
2014-10-29
影响因子：
4.5
通讯作者：
Tanaka K
中科院分区：
文献类型：
--
作者：
Nagata Y;Noguchi Y;Tamaru S;Kuwahara K;Okamoto A;Suruga K;Koba K;Tanaka K
我们之前的研究表明，无论胆固醇含量相对较高，鱿鱼匀浆都能降低动物的血清和肝脏胆固醇。自这项工作以来，我们开发了一种新的方法来抑制柠檬酸钠对鱿鱼蛋白的自溶。本研究旨在从分子水平探讨柠檬酸钠制备的鱿鱼匀浆对SD大鼠脂代谢的影响。为了抑制鱿鱼蛋白的自溶，我们用柠檬酸钠制备了鱿鱼匀浆。在实验1(实验1)中1)，给予大鼠无胆固醇对照饲料或鱿鱼饲料，添加5%的鱿鱼匀浆作为饲料蛋白，连续4周。禁食6小时后取血、肝脏和脂肪组织。测定大鼠血清、肝脂及脂代谢相关酶活性。在实验2中(实验22)上述饲料按0.1%的水平添加胆固醇，给大鼠灌胃。测定肝脏和小肠的脂类参数、酶活性以及与脂类代谢有关的蛋白质的基因表达。此外，在实验结束时收集两天的粪便。2测定粪便类固醇排泄量。在实验1中，喂鱿鱼饲料的大鼠血清甘油三酯和胆固醇分别比对照组低50%和20%，而肝脏胆固醇比喂鱿鱼饲料的大鼠高出约290%。当饮食中包含胆固醇时(实验2)鱿鱼组大鼠血脂水平显著低于对照组，而肝脂含量两组间无明显差异。食用鱿鱼饲料的大鼠肝脏成脂酶活性显着降低，而负责脂肪酸氧化的酶却没有被修饰(Expt。1和2)。鱿鱼组大鼠肝脏微粒体甘油三酯转运蛋白的表达水平显著低于对照组。在小肠中，鱿鱼饲料中与脂肪酸运输和胆固醇吸收相关的蛋白质的基因表达显著降低。与对照组相比，喂食鱿鱼饲料的大鼠粪便中酸性类固醇的分泌量更高，但中性类固醇的分泌量却没有增加。这些结果表明，新开发的鱿鱼匀浆具有降血脂的潜力，主要是通过减少小肠对胆汁酸的吸收和抑制肝脏的脂肪生成。
Our previous study has shown that regardless of a relatively high amount of cholesterol, squid homogenate lowers serum and hepatic cholesterol in animals. Since this work, we have developed a new method to inhibit autolysis of squid proteins with sodium citrate. This study aims to investigate how squid homogenate prepared with sodium citrate affects lipid metabolism in Sprague–Dawley rats at the molecular level. We prepared squid homogenate with sodium citrate to inhibit autolysis of squid protein. In Experiment 1 (Exp. 1), rats were given a cholesterol-free control diet or a squid diet, with squid homogenate added at the level of 5% as dietary protein for 4 weeks. Blood, the liver and adipose tissue were taken after 6 hours fasting. Serum and hepatic lipids and activities of enzymes related to lipid metabolism were measured. In Experiment 2 (Exp. 2), the above-mentioned diets had cholesterol added at the level of 0.1% and given to rats. Lipid parameters, enzyme activities, and gene expression of proteins involved in lipid metabolism in the liver and the small intestine were determined. In addition, feces were collected for two days at the end of Exp. 2 to measure fecal excretion of steroids. In Exp.1, serum triglyceride and cholesterol were ~50% and ~20% lower, respectively, in the squid diet-fed rats than in the control diet-fed animals while hepatic cholesterol was ~290% higher in the squid diet-fed rats. When cholesterol was included into the diets (Exp. 2), serum lipids were significantly lower in the squid group while no difference of hepatic lipid was seen between two groups. Activities of hepatic lipogenic enzymes were significantly lower in rats on the squid diet while the enzyme responsible for fatty acid oxidation was not modified (Expt. 1 and 2). Hepatic level of mRNA of microsomal triglyceride transfer protein was significantly lower in the squid group. In the small intestine, the squid diet exhibited significantly lower gene expression of proteins involved in fatty acid transport and cholesterol absorption. Fecal secretion of acidic steroids, but not neutral steroids, was higher in rats fed the squid diet than in those fed the control diet. These results imply that newly-developed squid homogenate has hypolipidemic potential primarily through decreased absorption of bile acids in the small intestine and suppressed lipogenesis in the liver.