Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry.
Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry.
复制标题
通过液相色谱串联质谱法分析人血浆中儿茶酚雌激素代谢物的数据。
DOI：
10.1016/j.dib.2019.103740
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发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Denver N
中科院分区：
文献类型：
--
作者：
Denver N
由于儿茶酚雌激素（2 和 4 羟基雌酮和雌二醇）浓度低、半衰期短且对温度不稳定，因此采用液相色谱串联质谱法对其进行分析非常困难。 “衍生化增强了通过液相色谱串联质谱法对人血浆中雌激素及其生物活性代谢物的分析”（Denver 等人，2019）中报道了一组 9 种雌激素衍生为甲基哌嗪类似物。数据显示，需要改变该方法中的基础催化剂才能检测到低水平的儿茶酚雌激素。数据还强调了异构体和同位素体的色谱分离所面临的挑战，通过采用延长的柱长度和降低的柱箱温度部分克服了这些挑战。此外，数据分析显示，固相萃取后，尽管回收率很高，但在血浆定量过程中仍存在显着的基质效应。
Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, “Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry” (Denver et al., 2019). Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were partially overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.