FijiWings: an open source toolkit for semiautomated morphometric analysis of insect wings.
FijiWings: an open source toolkit for semiautomated morphometric analysis of insect wings.
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DOI：
10.1534/g3.113.006676
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发表时间：
2013-08-07
期刊：
影响因子：
--
通讯作者：
Dobens LL
中科院分区：
文献类型：
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作者：
Dobens AC;Dobens LL
发育需要细胞增殖和细胞生长之间的协调，以形成组织、器官和整个生物体的适当大小。果蝇翅膀具有标志性特征，例如由细胞群图案化的静脉位置和单个细胞产生的毛状体结构，这些特征有助于检查遗传对组织和细胞大小的贡献。翅膀的大小和毛状体密度是手动测量的，这是繁琐且容易出错的，尽管图像处理和模式识别软件可以量化显微照片中的特征，但这种方法尚未应用于昆虫翅膀。在这里，我们介绍 FijiWings，这是一组旨在对机翼显微照片进行半自动形态光度分析的宏。 FijiWings 使用 ImageJ 斐济版本中安装的插件来检测和计数毛状体并测量机翼面积，以计算用户选择的定义区域的毛状体密度或生成总体毛状体密度的热图。对于高通量屏幕，我们开发了一个宏，可指导可训练的分割插件来检测翼静脉位置，以测量特定静脉间区域的毛状体密度或生成相对静脉间区域的热图。我们使用翼 GAL4 驱动程序和 UAS 调节的转基因来确认这些工具检测整体组织生长和个体细胞大小变化的能力。 FijiWings 是免费提供的，并且会引起广泛的果蝇遗传学家的兴趣，他们研究基因功能对翅膀图案的影响和翅膀形态的进化。
Development requires coordination between cell proliferation and cell growth to pattern the proper size of tissues, organs, and whole organisms. The Drosophila wing has landmark features, such as the location of veins patterned by cell groups and trichome structures produced by individual cells, that are useful to examine the genetic contributions to both tissue and cell size. Wing size and trichome density have been measured manually, which is tedious and error prone, and although image processing and pattern-recognition software can quantify features in micrographs, this approach has not been applied to insect wings. Here we present FijiWings, a set of macros designed to perform semiautomated morphophometric analysis of a wing photomicrograph. FijiWings uses plug-ins installed in the Fiji version of ImageJ to detect and count trichomes and measure wing area either to calculate trichome density of a defined region selected by the user or generate a heat map of overall trichome densities. For high-throughput screens we have developed a macro that directs a trainable segmentation plug-in to detect wing vein locations either to measure trichome density in specific intervein regions or produce a heat map of relative intervein areas. We use wing GAL4 drivers and UAS-regulated transgenes to confirm the ability of these tools to detect changes in overall tissue growth and individual cell size. FijiWings is freely available and will be of interest to a broad community of fly geneticists studying both the effect of gene function on wing patterning and the evolution of wing morphology.