Synthesis and Characterization of Highly Thiolated Silk Fibroin
Synthesis and Characterization of Highly Thiolated Silk Fibroin
复制标题
高硫醇化丝素蛋白的合成与表征
DOI：
10.1002/macp.202300340
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发表时间：
2023
影响因子：
2.5
通讯作者：
Murphy, Amanda R.
中科院分区：
文献类型：
--
作者：
Talusig, Jeremy M.;Murphy, Amanda R.
通过含吡啶基二硫醇的N-羟基琥珀酰亚胺酯和氨基酪氨酸丝中间体的反应，以高度官能化实现硫醇与丝素蛋白的酪氨酸残基的共价连接。用1H NMR和UV-vis光谱表征了巯基修饰的程度。硫醇基团的进一步修饰通过与含碘乙酰胺的小分子反应来探测，从而产生新型荧光丝衍生物。最后，研究了巯基化蚕丝原位形成水凝胶的能力。
Covalent attachment of thiols to the tyrosine residues of silk fibroin is accomplished with a high degree of functionalization through the reaction of a pyridyldithiol‐containing N‐hydroxysuccinimidal‐ester and an amino‐tyrosine silk intermediate. The extent of thiol modification is characterized by1H NMR and UV–vis spectroscopy. Further modification of the thiol groups is probed by reacting with an iodoacetamide‐containing small molecule resulting in a novel fluorescent silk derivative. Last, the ability of the thiolated silk to form hydrogels in situ is investigated.