Identification of target antigens of antiendothelial cell antibodies in healthy individuals: A proteomic approach
Identification of target antigens of antiendothelial cell antibodies in healthy individuals: A proteomic approach
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健康个体抗内皮细胞抗体靶抗原的鉴定：蛋白质组学方法
DOI：
10.1002/pmic.200700794
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发表时间：
2008
期刊：
影响因子：
3.4
通讯作者：
L. Mouthon
中科院分区：
文献类型：
--
作者：
A. Servettaz;P. Guilpain;L. Camoin;P. Mayeux;C. Broussard;M. Tamby;N. Tamas;S. Kaveri;L. Guillevin;L. Mouthon
In order to identify target antigens of anti‐endothelial cell (anti‐EC) antibodies (AECA) in healthy individuals, we have used a proteomic approach combining 2‐DE and immunoblotting. Whole cell protein extracts obtained from human umbilical vein EC (HUVEC) cultures were used as a source of antigens. Serum IgG from 12 healthy blood donors were tested at a concentration of 200 μg/mL. Targeted spots were identified by MS. The HUVEC proteome was composed of 884 protein spots. Among these, 61 ± 25.8 (mean ± SD) spots were recognized by serum IgG from healthy individuals, with marked differences from one individual to another. Among these spots, 11 were recognized by serum IgG from all healthy individuals tested. These spots corresponded to six different proteins with several spots corresponding to different isoforms of the same protein. Target antigens were: cytoskeletal proteins (β‐actin, α‐tubulin, and vimentin); glycolytic enzymes (glucose‐3‐phosphate‐deshydrogenase and α‐enolase); and prolyl‐4‐hydroxylase β subunit, a member of the disulfide isomerase family. This study shows that the repertoire of IgG AECA is heterogeneous among healthy individuals. IgG from all of the healthy individuals tested recognized a restricted set of highly conserved ubiquitous proteins playing key roles in cell biology and maintenance of homeostasis.
自身抗体：术语和概念。
DOI：
10.1016/s0090-1229(88)80002-3
发表时间：
1988
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Francoeur,AM;Heitzmann,JG
通讯作者：
Heitzmann,JG