Effects of Wheat-Faba Bean Intercropping on Soil Microbial Community Structure in the Rhizosphere
Effects of Wheat-Faba Bean Intercropping on Soil Microbial Community Structure in the Rhizosphere
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DOI：
10.4236/as.2018.911096
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Yongbo Xu;B. Lei;Ya-Fang Tang
中科院分区：
文献类型：
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作者：
Yongbo Xu;B. Lei;Ya-Fang Tang
采用磷脂脂肪酸（PLFA）分析和实时荧光定量PCR（real-time quantitative PCR）技术，研究了小麦蚕豆间作对红壤收获期根际土壤微生物群落及氨氧化古菌（AOA）和细菌（AOB）氨单加氧酶（amoA）基因丰度的影响。结果表明，收获期蚕豆根际细菌、真菌和小麦根际放线菌数量在间作和单作间差异显著（p < 0.05）。收获期根际共检测到37种PLFA，其中细菌PLFA 31种，真菌PLFA 3种，放线菌PLFA 3种。收获期间作蚕豆根际AOB丰度低于单作蚕豆根际，单作小麦和间作小麦根际AOB丰度差异不显著。单作和间作根际AOA丰度差异不显著，但间作系统中AOA丰度较高，间作后根际AOB丰度显著高于AOA。研究结果表明，小麦蚕豆间作可能会改变根际微环境和微生物群落结构。
Phospholipid fatty acids (PLFA) analysis and real-time quantitative PCR were used to investigate the effect of wheat-faba bean intercropping on soil microbial community in the rhizosphere and ammonia monooxygenase (amoA) gene abundances of ammonia-oxidizing archaea (AOA) and bacteria (AOB) at harvest stage via field trials in the red soil. We found that the bacteria and fungi of faba bean and actinomycetes of wheat in the rhizosphere showed significant (p < 0.05) difference between intercrops and monocrops at harvest. In total, 37 PLFA were detected at harvest stage in the rhizosphere, including 31 bacterial PLFA, 3 fungal PLFA, and 3 actinomycete PLFA. Compared with the rhizosphere of monocropped faba bean, a lower AOB abundance was found in the intercropping at harvest stage, whereas no significant difference in the AOB abundance was found in the rhizosphere of monocropped and intercropped wheat. There was no significant difference in the AOA abundance between monocrops and intercrops rhizosphere, but a higher AOA abundance in the intercropping systems was found. After intercropping, the abundance of AOB in rhizosphere was significantly higher than that of AOA. Our findings suggest that wheat-faba bean intercropping may change the micro-environment and microbial community structure in the rhizosphere.