Lamina lucida of basement membrane: An artefact
Lamina lucida of basement membrane: An artefact
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基底膜透明层：人工制品
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
S. Inoue
中科院分区：
文献类型：
--
作者：
F. Chan;S. Inoue
在化学固定后常规脱水制备的组织中，观察到基底膜是由透明层和致密层以及被称为纤维网状部的有限过渡区组成的层压结构。近年来，在应用新的组织制备技术，如冷冻固定或冷冻置换来研究基底膜结构方面进行了零星的尝试。根据这些研究，基底膜可能只由致密层组成，而没有透明层。在本实验室最近的研究中，试图确定这个透明板是否是人工制品，如果是的话，在传统的组织制备方法中的哪一步是其形成的原因。在通过slam冷冻然后冷冻置换或醛固定然后冷冻置换进行冷冻固定后，观察了小鼠和大鼠不同来源的基底膜，包括睾丸、附睾管、眼、甲状腺、肾脏和皮肤。两种方法保存后的基底膜仅由致密层组成，无透明层。这表明，在传统的组织制备过程中，而不是在化学固定过程中脱水时，会出现透明板的伪影形成。鉴于众所周知的冷冻置换优于常规脱水，基底膜的透明层很可能是人工制品。因此，可以得出结论，透明板是在常规组织制备过程中形成的人工制品，并且在其在活状态下的原始条件下，基底膜由致密板材料构成的单层组成。© 1994 Wiley利斯公司
In tissues prepared with chemical fixation followed by conventional dehydration, basement membranes have been observed to be laminated structures composed of a lamina lucida and lamina densa as well as a poorly limited transitional zone referred to as the pars fibroreticularis. Scattered attempts in the application of new techniques of tissue preparation such as cryofixation or freeze substitution for the study of the basement membrane structure have been made in recent years. From these studies, the possibility has arisen in which basement membranes are composed of only the lamina densa without a lamina lucida. In recent studies in this laboratory, the attempt was made to determine whether or not this lamina lucida is an artefact, and if so, which step in the conventional method of tissue preparation is responsible for its formation. Basement membranes from diverse sources in the mouse and rat including the testis, ductus epididymis, eye, thyroid, kidney, and skin, were observed after either cryofixation by slam freezing followed by freeze substitution, or aldehyde fixation followed by freeze substitution. The basement membranes after preservation with either of these two methods were composed of only the lamina densa with no lamina lucida. It indicates that an artefactual formation of the lamina lucida occurs during dehydration in conventional tissue preparation rather than during chemical fixation. In view of the well known superiority of freeze substitution over conventional dehydration, the lamina lucida of the basement membrane is likely to be an artefact. Therefore, it is concluded that the lamina lucida is an artefact formed during conventional tissue preparation, and in its original condition in the living state, the basement membrane is composed of a single layer made up of lamina densa material. © 1994 Wiley‐Liss, Inc.
DOI：
10.1002/aja.1001810403
发表时间：
1988
期刊：
The American journal of anatomy
影响因子：
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作者：
Inoue,S;Leblond,CP
通讯作者：
Leblond,CP
影响因子：
--
作者：
LEBLOND, CP;INOUE, S
通讯作者：
INOUE, S
基底膜的超微结构。
DOI：
10.1016/s0074-7696(08)61334-0
发表时间：
1989
期刊：
International review of cytology
影响因子：
--
作者：
Inoue,S
通讯作者：
Inoue,S