Matrix metalloproteinase gene polymorphisms: lack of association with chronic obstructive pulmonary disease in a Brazilian population
Matrix metalloproteinase gene polymorphisms: lack of association with chronic obstructive pulmonary disease in a Brazilian population
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DOI：
10.4238/vol8-3gmr596
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发表时间：
2009-01-01
影响因子：
0.4
通讯作者：
Simon, D.
中科院分区：
文献类型：
--
作者：
Schirmer, H.;da Silva, L. Basso;Simon, D.
慢性阻塞性肺疾病（COPD）有许多候选基因。其中一个候选基因是一组编码基质金属蛋白酶（MMP）的基因，它们在与慢性阻塞性肺病相关的组织重塑和修复中发挥着重要作用。我们通过检查 111 名 COPD 患者和 101 名对照者的 MMP-3、MMP-9 和 MMP-12 基因启动子的功能多态性，检验了 MMP 基因的多态性变异影响患 COPD 风险的假设。通过聚合酶链反应和限制性消化分析-1171 5A/6A MMP-3、-1562 C/T MMP-9 和-82 A/G MMP-12 多态性。 COPD 患者和对照组之间的等位基因和基因型频率没有观察到显着差异。单倍型分析也没有揭示 COPD 患者和对照组之间的差异。我们发现 MMP 多态性对巴西样本中患 COPD 的风险没有显着影响。
There are many candidate genes for chronic obstructive pulmonary disease (COPD). One such candidate is the group of genes that code for matrix metalloproteinases (MMPs), which play an essential role in tissue remodeling and repair associated with COPD. We tested the hypothesis that polymorphic variation in MMP genes influences the risk of developing COPD by examining functional polymorphisms in the promoters of MMP-3, MMP-9 and MMP-12 genes in 111 COPD patients and 101 controls. The -1171 5A/6A MMP-3, -1562 C/T MMP-9 and -82 A/G MMP-12 polymorphisms were analyzed by polymerase chain reaction, followed by restriction digestion. No significant differences were observed in allele and genotype frequencies between COPD patients and controls. Haplotype analysis also did not reveal differences between COPD patients and controls. We found that MMP polymorphisms had no significant impact on the risk of developing COPD in this Brazilian sample.