The Rv1651c-encoded PE-PGRS30 protein expressed in Mycobacterium smegmatis exhibits polar localization and modulates its growth profile
The Rv1651c-encoded PE-PGRS30 protein expressed in Mycobacterium smegmatis exhibits polar localization and modulates its growth profile
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DOI：
10.1111/j.1574-6968.2011.02354.x
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Garg, Lalit C.
中科院分区：
文献类型：
--
作者：
Chatrath, Shweta;Gupta, Vineet Kumar;Garg, Lalit C.
结核分枝杆菌（Mtb）H37Rv的全基因组测序分析导致一个新的多基因，PE基因家族的鉴定。PE家族中最大的PE_PGRS亚家族的基因主要限于致病性分枝杆菌，并且它们在Mtb生物学中的确切作用尚不清楚。基于它们的序列同源性，PE_PGRS蛋白最初被认为具有共同的功能。然而，对单个蛋白质的研究表明，这个亚家族的单个蛋白质可能执行几个不相关的任务。本研究通过在耻垢分枝杆菌中表达PE_PGRS30来研究其功能。PE_PGRS30在M.斯美克灵导致表型变化，菌落形态和生长曲线改变。重组质粒PE_PGRS30具有极性定位，并与M.恶臭因此，本研究表明，由PE_PGRS 30引起的生长延迟期延长可能部分地导致Mtb的潜伏期。
Sequencing analysis of the complete genome of Mycobacterium tuberculosis (Mtb) H37Rv resulted in the identification of a novel multigene, the PE family of genes. The genes of the largest PE_PGRS subfamily of the PE family are mainly restricted to pathogenic mycobacteria, and their exact role in the biology of Mtb is not clearly understood. Based on their sequence homology, PE_PGRS proteins were initially thought to serve common functions. However, studies on individual proteins reveal that the individual proteins of this subfamily could be performing several unrelated tasks. In the present study, we investigated the function of PE_PGRS30 by expressing it in Mycobacterium smegmatis. PE_PGRS30 expression in M. smegmatis resulted in phenotypic changes with altered colony morphology and growth profile. The recombinant PE_PGRS30 showed polar localization and was found to be associated with the cell wall of M. smegmatis. Thus, the present study suggests that the prolonged lag phase of growth caused by the PE_ PGRS30 may, in part, contribute to the latency of Mtb.