T cell help in cytotoxic T lymphocyte responses. Role of the I region in helper cell induction.
T cell help in cytotoxic T lymphocyte responses. Role of the I region in helper cell induction.
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T 细胞有助于细胞毒性 T 淋巴细胞反应。
DOI：
10.1097/00007890-198301000-00015
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发表时间：
1983
期刊：
影响因子：
6.2
通讯作者：
Corley,RB
中科院分区：
文献类型：
--
作者：
Livnat,S;Corley,RB
我们研究了体内诱导参与细胞毒性T淋巴细胞生成的辅助T细胞（T H）。辅助活性是通过细胞帮助静止胸腺T细胞前体在体外发展成效应T细胞的能力来测量的。将同种异体脾细胞直接注入小鼠足垫，可在4 ~ 6天内在腘窝引流淋巴结内产生同种异体抗原特异性辅助细胞。辅助活性由尼龙-羊毛非粘附Lyt-1+ T淋巴细胞介导；一些活性与lyt - 1,2 +细胞有关。利用H- 2k /H- 2d重组小鼠细胞作为体内免疫原和体外刺激剂，研究了诱导和再刺激C3H抗H- 2d T细胞的遗传条件。有证据表明，在直接测定中，T - H细胞本身对I区编码决定因子具有特异性。因此，H-2复合体左侧的差异（K到IE），而不仅仅是H- 2k的差异，是诱导和重新激活T - H细胞的必要和充分条件。混合淋巴细胞培养中的增殖是在T - H细胞检测不到的组合中测量的，这支持了增殖不能被严格认为是辅助细胞的测量的观点。
We have studied the in vivo induction of T helper (T H) cells that participate in the generation of cytotoxic T (T c) lymphocytes. Helper activity was measured by the ability of the cells to help resting thymic T c cell precursors develop into effector T c cells in vitro. Direct injection of allogeneic spleen cells into the footpads of mice led to the generation of alloantigen-specific helper cells in the draining popliteal lymph nodes within 4 to 6 days. Helper activity was mediated by nylon-wool-nonadherent Lyt-1+ T lymphocytes; some activity was associated with Lyt-1, 2+ cells. The genetic requirements for both the induction and restimulation of C3H anti-H-2 d T H cells were investigated using cells from H-2 k/H-2 d recombinant mice as in vivo immunogens and in vitro stimulators. Evidence is presented that shows in a direct assay that T H cells themselves are specific for I region-coded determinants. Thus, disparity at the left side of the H-2 complex (K to IE) but not at H-2K alone was necessary and sufficient to induce and reactivate T H cells. Proliferation in mixed lymphocyte culture was measured in combinations in which T H cells were not detectable, supporting the idea that proliferation cannot be strictly considered a measurement of helper cells.