Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women.
Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women.
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DOI：
10.1093/oxfordjournals.humrep.a019080
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发表时间：
1996-10
影响因子：
6.1
通讯作者：
David E. Battaglia;P. Goodwin;Nancy A. Klein;M. Soules
中科院分区：
文献类型：
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作者：
David E. Battaglia;P. Goodwin;Nancy A. Klein;M. Soules
为了研究母体衰老对减数分裂装置的影响，我们从两个年龄组的自然周期女性中获得卵母细胞，包括年轻（20-25岁）和老年（40-45岁）女性。使用高分辨率共聚焦显微镜，我们获得了详细的图片减数分裂纺锤体和染色体的位置在减数分裂的各个阶段。我们的数据显示，在老年妇女的减数分裂纺锤体是经常异常的，无论是关于染色体排列和微管矩阵，构成减数分裂纺锤体。在第二次减数分裂过程中，79%的老年组卵母细胞的纺锤体表现出异常的微管蛋白位置，一条或多条染色体从中期板上移位。相比之下，只有17%的卵母细胞从较年轻的年龄组表现出非整倍体的条件。这一组中的大多数卵子具有良好有序的减数分裂纺锤体，其中含有在纺锤体中的不同中期板内完全对齐的染色体。减数分裂期间的染色体管理由纺锤体内的微管组装指导。这些数据表明，老年女性中负责减数分裂纺锤体组装的调节机制发生了显着改变，导致非整倍体的高患病率。
To examine the effects of maternal ageing on the meiotic apparatus, we obtained oocytes from naturally cycling women in two age groups, including younger (aged 20-25 years) and older (aged 40-45 years) women. Using high-resolution confocal microscopy we obtained a detailed picture of the meiotic spindle and chromosome placement during various phases of meiosis. Our data revealed that the meiotic spindle in older women is frequently abnormal, both with regard to chromosome alignment and the microtubule matrix that comprise the meiotic spindle. The spindle in 79% of the oocytes from the older group exhibited abnormal tubulin placement and one or more chromosomes were displaced from the metaphase plate during the second meiotic division. In contrast, only 17% of the oocytes from the younger age group exhibited aneuploid conditions. The majority of eggs from this group possessed a well ordered, meiotic spindle containing chromosomes that were fully aligned within a distinct metaphase plate in the spindle. Chromosome management during meiosis is directed by microtubule assembly within the spindle. These data suggest that the regulatory mechanisms responsible for assembly of the meiotic spindle are significantly altered in older women, leading to the high prevalence of aneuploidy.