The role of porcine reproductive and respiratory syndrome virus infection in immune phenotype and Th1/Th2 balance of dendritic cells
The role of porcine reproductive and respiratory syndrome virus infection in immune phenotype and Th1/Th2 balance of dendritic cells
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猪繁殖与呼吸综合征病毒感染对树突状细胞免疫表型及Th1/Th2平衡的影响
DOI：
10.1016/j.dci.2016.07.012
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发表时间：
2016-12-01
影响因子：
2.9
通讯作者：
Shen, Guoshun
中科院分区：
文献类型：
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作者：
Liu, Jinling;Wei, Shu;Shen, Guoshun
本研究的目的是表征猪外周血中来源于单核细胞的树突状细胞（Mo-DCs）感染猪繁殖与呼吸综合征病毒（PRRSV）后的免疫应答。病毒载量检测结果表明，PRRSV感染Mo-DCs后，其SLA-I、SLA-II、CD 80和CD 40的表达均明显降低。此外，我们分析了细胞因子谱采用定量RT-PCR和ELISA。结果表明，在感染PRRSV的Mo-DCs和未感染的Mo-DCs中，IL-10和IL-12 p40表达有明显变化，但IFN-γ和TNF-α无明显变化。经IL-4和GM-CSF诱导7天后，流式细胞仪分析显示，在感染PRRSV前，Mo-DCs具有典型的形态和表型，纯度为91.73%。总之，我们的数据表明，PRRSV损害了Mo-DCs的正常抗原呈递，并通过下调SLA-I、SLA-II、CD 80和CD 40的表达导致适应性免疫应答不足。Th 2型细胞因子IL-10分泌增加和Th 1型细胞因子IL-12 p40、IFN-γ和TNF-α分泌减少导致Th 1/Th 2失衡。(C)2016爱思唯尔有限公司版权所有
The aim of this study was to characterize the immune response of dendritic cells derived from monocytes (Mo-DCs) in the porcine, peripheral blood following infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral load assays indicated that PRRSV efficiently infected Mo-DCs but failed to replicate, whereas PRRSV infection of Mo-DCs decreased the expression of SLA-I, SLA-II, CD80 and CD40 compared with those of mock Mo-DCs. Furthermore, we analyzed the cytokine profiles using quantitative RT-PCR and ELISA. Results indicated apparent changes in IL-10 and IL-12 p40 expression but not in IFN-gamma and TNF-alpha among Mo-DCs infected with PRRSV and uninfected Mo-DCs. Additionally, flow cytometry analysis of the altered Mo-DCs together with IL-4 and GM-CSF induction for 7days revealed the typical morphology and phenotype with 91.73% purity before infection with PRRSV. Overall, our data demonstrate that PRRSV impaired the normal antigen presentation of Mo-DCs and led to inadequate adaptive immune response by down-regulating the expression of SLA-I,SLA-II, CD80 and CD40. Enhanced Th2-type cytokine IL-10 secretion and reduced Th1-type cytokines IL-12p40,IFN-gamma and TNF-alpha secretion results in Th1/Th2 imbalance. (C) 2016 Elsevier Ltd. All rights reserved.