Comparative studies of paeoniflorin and albiflorin from Paeonia lactiflora on anti-inflammatory activities
Comparative studies of paeoniflorin and albiflorin from Paeonia lactiflora on anti-inflammatory activities
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芍药中芍药苷和白花苷抗炎活性的比较研究
DOI：
10.3109/13880209.2014.880490
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发表时间：
2014-09-01
影响因子：
3.8
通讯作者：
Jiang, Heng-Li
中科院分区：
文献类型：
--
作者：
Wang, Qiang-Song;Gao, Teng;Jiang, Heng-Li
摘要背景：野牡丹Pall。（毛茛科）已被用于超过1000年的中医治疗妇科问题，抽筋，疼痛，头晕，充血。芍药苷是从芍药中分离得到的单萜苷类化合物，具有多种药理活性。然而，另一种主要的单萜苷--芍药苷的药理活性尚未得到很好的研究。目的：采用脂多糖（LPS）诱导的RAW 264.7细胞模型，研究芍药苷和芍药内酯苷的抗炎活性。材料与方法：用Griess比色法测定一氧化氮（NO）的产生。此外，采用酶联免疫吸附试验（ELISA）分析前列腺素E2（PGE 2）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的合成。ELISA法检测细胞内环氧合酶-2（考克斯-2）蛋白的表达。实时荧光定量逆转录聚合酶链反应（real-time RT-PCR）检测诱导型一氧化氮合酶（iNOS）、考克斯-2、TNF-α和IL-6基因表达水平。结果如下：与LPS诱导组相比，芍药苷对NO、PGE 2、TNF-α和IL-6产生的抑制率分别为17.61、27.56、20.57和29.01%，芍药内酯苷为17.35、12.94、15.29和10.78%。芍药苷和芍药内酯苷对NO生成的IC 50值分别为2.2 × 10−4 mol/L和1.3 × 10−2 mol/L。芍药苷和芍药内酯苷分别使考克斯-2蛋白表达降低50.98%和17.21%。芍药苷对iNOS、考克斯-2、IL-6和TNF-α基因表达的抑制率分别为35.65%、38.08%、19.72%和45.19%;芍药内酯苷对iNOS、COX-2、IL-6和TNF-α基因表达的抑制率分别为58.36%、47.64%、50.70%和12.43%。结论：结果表明，芍药内酯苷具有与芍药苷相似的抗炎作用，为芍药内酯苷作为新的化学标记物用于质量控制和《中国药典》修订提供了新的证据。
Abstract Context: Paeonia lactiflora Pall. (Ranunculaceae) has been used for more than 1000 years in traditional Chinese medicine for the treatment of gynecological problems, cramp, pain, giddiness, and congestion. Paeoniflorin, monoterpene glycosides isolated from P. lactiflora, possesses a variety of pharmacological activities. However, the pharmacological activity of the pharmacological activity of albiflorin, another main monoterpene glycoside, has not been well studied. Objectives: The present study investigated the anti-inflammatory activities of paeoniflorin and albiflorin using models of lipopolysaccharides (LPS) induced RAW 264.7 cells. Materials and methods: Production of nitric oxide (NO) was measured by the Griess colorimetric method. In addition, prostaglandin E2 (PGE2), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) synthesis were analyzed using an enzyme-linked immunosorbent assay (ELISA). The protein expression of cyclooxygenase-2 (COX-2) was detected by a cell-based ELISA. The gene expression levels of inducible nitric oxide synthase (iNOS), COX-2, TNF-α, and IL-6 were detected by quantitative real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). Results: Compared with the LPS-induced group, the inhibition rates of NO, PGE2, TNF-α, and IL-6 production were 17.61, 27.56, 20.57, and 29.01% by paeoniflorin and 17.35, 12.94, 15.29, and 10.78% by albiflorin. The IC50 values of paeoniflorin and albiflorin on NO production were 2.2 × 10−4 mol/L and 1.3 × 10−2 mol/L, respectively. The protein expression of COX-2 was reduced by 50.98% with paeoniflorin and 17.21% with albiflorin. The inhibition rates of gene expression of iNOS, COX-2, IL-6, and TNF-α were 35.65, 38.08, 19.72, and 45.19% by paeoniflorin and 58.36, 47.64, 50.70, and 12.43% by albiflorin, respectively. Conclusion: These results show that albiflorin has similar anti-inflammatory effects to paeoniflorin, which provides new evidence that albiflorin can serve as a new chemical marker for the quality control of Paeoniae Radix and the Chinese Pharmacopoeia can be updated.