Efficient extraction and sensitive LC-MS quantification of hydroxytyrosol in wine, oil and plasma
Efficient extraction and sensitive LC-MS quantification of hydroxytyrosol in wine, oil and plasma
复制标题
葡萄酒、油和血浆中羟基酪醇的高效提取和灵敏的 LC-MS 定量
DOI：
10.1016/j.foodchem.2020.126803
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Yan Li
中科院分区：
文献类型：
--
作者：
Shao-Ting Wang;Juan Le;Rui Peng;Yan Li
羟基酪醇（HT）具有重要的生物活性。但其定量方法存在灵敏度低、处理复杂、样品消耗量大等问题。在这里，我们提出了第一次尝试的具体提取HT通过顺式二醇识别机制。以易于制备的二氧化锆为分散固相萃取介质，在10 min内即可从少量酒（10 μL）、油（20 mg）和血浆（100 μL）中有效地分离纯化HT。结合LC-MS/MS分析，方法的检测限（LOD）在葡萄酒中可达1 ng/mL，在油中可达0.5 μg/kg，在血浆中可达0.1 ng/mL。得益于这种上级方法，HT分析成功地在不同的葡萄酒和油产品以及摄入特级初榨橄榄油后的人血浆样品中进行。此外，我们进一步证实，即使在通过异烟酰氯衍生后提高检测灵敏度后，也无法从常规人血浆中检测到内源性HT。
Hydroxytyrosol (HT) possesses significant biological activity. However, the methodologies for its quantification always suffered from low sensitivity, intricate treatment and high sample consumption. Here, we presented the very first attempt for specific extraction of HT through cis-diol recognition mechanism. By using easily prepared zirconia as dispersive solid phase extraction medium, HT from small amount of wine (10 μL), oil (20 mg) and plasma (100 μL) was efficiently purified within ten minutes. Coupled with LC-MS/MS analysis, the method limit of detection (LOD) could reach 1 ng/mL in wine, 0.5 μg/kg in oil and 0.1 ng/mL in plasma. Profited by this superior method, HT analysis was successfully performed in diverse wine and oil products as well as human plasma samples after intake of extra virgin olive oil. In addition, we further confirmed the endogenous HT was undetectable from routine human plasma even after upgrading the detection sensitivity through post isonicotinoyl chloride derivatization.