PPR protein Early Chloroplast Development 2 is essential for chloroplast development at the early stage of Arabidopsis development
PPR protein Early Chloroplast Development 2 is essential for chloroplast development at the early stage of Arabidopsis development
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DOI：
10.3389/fpls.2020.01198
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jianwei Xiao（肖建伟）
中科院分区：
文献类型：
--
作者：
Xinwei Wang;Yaqi Wang;Zhi Qi;Jianwei Xiao（肖建伟）
Chloroplast biogenesis and development regulation have long been a focus of research; however, the underlying.mechanisms of these processes have not yet been fully elucidated. Pentatricopeptide repeat (PPR) proteins have.been shown to play key roles in chloroplast development. Here, we identified a novel P-type PPR protein, Early.Chloroplast Development 2 (ECD2), and the ecd2 mutant resulted in embryo lethality. The RNAi lines of ECD2.showed varying degrees of albino cotyledons and abnormal chloroplast development, but true leaves were.similar to the wild-type. Further analysis revealed that ECD2 was responsible for chloroplast gene expression and.group II intron splicing of several genes. Transcriptome analysis combined with quantitative real-time PCR.showed that ECD2 was associated with the expression of ribosomal genes and accumulation of chloroplast ribosomes..Overall, our results indicate that ECD2 is critically important for early chloroplast development in.cotyledon.
