The Periodontopathogenic Bacterium Eikenella corrodens Produces an Autoinducer-2-Inactivating Enzyme
The Periodontopathogenic Bacterium Eikenella corrodens Produces an Autoinducer-2-Inactivating Enzyme
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DOI：
10.1271/bbb.130047
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发表时间：
2013-05
期刊：
影响因子：
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通讯作者：
M. M. Karim-M.;A. Nagao;F. J. Mansur;Tetsuro Matsunaga;Y. Akakabe;Y. Noiri;S. Ebisu;A. Kato;H. Azakami
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文献类型：
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作者：
M. M. Karim-M.;A. Nagao;F. J. Mansur;Tetsuro Matsunaga;Y. Akakabe;Y. Noiri;S. Ebisu;A. Kato;H. Azakami
艾肯氏菌腐蚀在测井中期产生自诱导剂-2 (AI-2)，在固定阶段AI-2活性急剧下降。我们研究了AI-2活性降低的机制。为了分析其作用机理，我们从中期培养的上清液中提取并纯化了AI-2。同时，采用硫酸铵沉淀法对固定相培养上清液进行分馏。将纯化的AI-2和4-羟基-5-甲基-3(2H)-呋喃酮（MHF）与各馏分孵育后，30%的馏分均降低了AI-2和MHF的活性。数据表明，AI-2和MHF以同样的方式失活。30%部分的热和/或胰蛋白酶处理不能完全阻止AI-2的失活，这表明部分热稳定蛋白参与了AI-2的失活。我们观察到一种酶将MHF转化为另一种形式。这表明E. corrosion产生了AI-2灭活酶，并且AI-2可以被它降解或修饰。
Eikenella corrodens produces autoinducer-2 (AI-2) in the mid log phase, and AI-2 activity decreases dramatically during the stationary phase. We investigated the mechanism underlying this decrease in AI-2 activity. To analyze the mechanism, we extracted and purified AI-2 from the supernatant of mid-log-phase culture. Simultaneously, the stationary-phase culture supernatant was fractionated by ammonium sulfate precipitation. On incubating purified AI-2 and 4-hydroxy-5-methyl-3(2H)-furanone (MHF) with each fraction, the 30% fraction decreased both AI-2 and MHF activities. The data suggest that AI-2 and MHF were rendered inactive in the same manner. Heat and/or trypsin treatment of the 30% fraction did not completely arrest AI-2 inactivation, suggesting that partially heat-stable proteins are involved in AI-2 inactivation. We observed that an enzyme converted MHF to another form. This suggests that E. corrodens produces an AI-2 inactivating enzyme, and that AI-2 can be degraded or modified by it.