The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots
The RETINOBLASTOMA-RELATED gene regulates stem cell maintenance in Arabidopsis roots
复制标题
DOI：
10.1016/j.cell.2005.09.042
复制
发表时间：
2005-12-29
期刊：
影响因子：
64.5
通讯作者：
Scheres, B
中科院分区：
文献类型：
--
作者：
Wildwater, M;Campilho, A;Scheres, B
干细胞在特定位置的维持对所有多细胞生物都是至关重要的。虽然干细胞命运的内在因素和信号已经在一些物种中被确定，但它们如何与干细胞重新进入细胞周期的能力联系起来仍然不清楚，而细胞周期是干细胞的定义特性之一。我们发现，拟南芥根部视网膜母细胞瘤相关（RBR）基因的局部表达减少会增加干细胞的数量，而不会影响有丝分裂活跃细胞的细胞周期持续时间。相反，诱导的RBR过表达会在抑制其他有丝分裂细胞之前消散干细胞。D cyclins、kip相关蛋白和E2F因子的过表达也会影响根干细胞池的大小，遗传相互作用表明这些因子在典型的RBR通路中发挥作用，调节体细胞干细胞。表达分析和基因相互作用定位rbr介导的干细胞状态调控位于模式基因稻草人的下游。
The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW.