Changes in microbial community composition following phytostabilization of an extremely acidic Cu mine tailings
Changes in microbial community composition following phytostabilization of an extremely acidic Cu mine tailings
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极酸性铜尾矿植物稳定后微生物群落组成的变化
DOI：
10.1016/j.soilbio.2017.07.004
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发表时间：
2017
影响因子：
9.7
通讯作者：
Li Jin-tian
中科院分区：
文献类型：
--
作者：
Yang Tao-tao;Liu Jun;Chen Wen-ce;Chen Xi;Shu Hao-yue;Jia Pu;Liao Bin;Shu Wen-sheng;Li Jin-tian
通过直接植被恢复实现极酸性（pH < 3）尾矿的植物稳定仍然是一个挑战，这可能主要归因于对极酸性尾矿中微生物群落对实际植物稳定的反应的了解有限。为了阐明植物稳定实践中使用的修正材料如何帮助改善重建根区生长条件，从而促进极酸性尾矿中植被的初步建立，在6个月内，在直接添加了改良剂的极酸性（pH 2.5）铜尾矿上成功建立了4000 m2的植被。对比分析了再生尾矿改质层（ALRT）、未改质层（ULRT）和未再生尾矿（UT）中的微生物群落。结果揭示了植物稳定后微生物群落组成的有趣变化。UT群落以酵母古细菌为主，相对丰度在57% ~ 84%之间，而在ULRT和ALRT群落中，proteobacteria是最占优势的门，相对丰度在29% ~ 53%之间。与尾矿酸化有关的两个古菌属ferroplasmaa和aplasma在UT群落中存在较高的相对丰度（高达52%），是ULRT和ALRT群落的14-913倍。ULRT的总体微生物群落组成与UT差异较大，但随着植物稳定的进行，有向ALRT转移的趋势。氧化还原电位是尾矿微生物群落组成的主要决定因素之一。这些发现对进一步开发有效的极酸性尾矿植物稳定方案具有指导意义。
Phytostabilization of an extremely acidic (pH < 3) mine tailings via direct revegetation remains a challenge, which can perhaps be attributed largely to a limited understanding of the responses of microbial communities in extremely acidic mine tailings to practical phytostabilization. In order to shed light on the mechanism by which amending materials used in phytostabilization practice help to improve the growth conditions in reconstructed root zones and thereby facilitate the initial establishment of vegetation in extremely acidic mine tailings, 4000 m2of vegetation was established successfully within six months on an extremely acidic (pH 2.5) Cu mine tailings amended directly with ameliorants. Further, a comparative analysis of the microbial communities in amended layer of the reclaimed tailings (ALRT), unamended layer of the reclaimed tailings (ULRT) and the unreclaimed tailings (UT) was performed. The results revealed intriguing changes in microbial community composition following the phytostabilization. While UT communities were dominated byEuryarchaeotawith relative abundances varying from 57% to 84%,Proteobacteriawas the most predominant phylum in those of both ULRT and ALRT with relative abundances ranging from 29% to 53%.FerroplasmaandAplasma, two archaeal genera involved in tailings acidification, existed in UT communities at high relative abundances (up to 52%), which were 14–913 times greater than those in ULRT and ALRT communities. The overall microbial community composition of ULRT was different greatly from that of UT, but tended to shift towards that of ALRT as the phytostabilization progressed. Redox potential was among the major determinants of microbial community composition of the tailings. These findings have implications for further development of effective phytostabilization schemes for extremely acidic mine tailings.