Infectious influenza A and B virus variants with long carboxyl terminal deletions in the NS1 polypeptides.
Infectious influenza A and B virus variants with long carboxyl terminal deletions in the NS1 polypeptides.
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DOI：
10.1016/0042-6822(87)90399-0
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发表时间：
1987-02
期刊：
影响因子：
3.7
通讯作者：
G. Norton;T. Tanaka;K. Tobita;S. Nakada;D. Buonagurio;D. Greenspan;M. Krystal;P. Palese
中科院分区：
文献类型：
--
作者：
G. Norton;T. Tanaka;K. Tobita;S. Nakada;D. Buonagurio;D. Greenspan;M. Krystal;P. Palese
蛋白质凝胶分析表明，甲型流感病毒A/土耳其/俄勒冈/71编码的NSI蛋白大约是其他甲型流感病毒的一半大小。对该病毒NS基因的序列分析显示，该病毒有10个核苷酸缺失，导致NS1蛋白只有124个氨基酸。病毒感染细胞的间接免疫荧光分析表明，截短的NS1多肽保留了其亲核模式。此外，A/土耳其/俄勒冈/71病毒在胚胎鸡蛋中生长到与其他甲型流感病毒相当的高滴度。我们还鉴定了B型流感病毒的一个实验室变种，克隆201，它编码一个截短的NS1蛋白。序列分析显示，NSI蛋白有13个核苷酸缺失，与其他B型流感病毒NS1蛋白相比，NSI蛋白仅缩短了127个氨基酸，而NS1蛋白的长度为281个氨基酸。同样，与其他B型流感病毒的NS1蛋白一样，B型病毒克隆201的NSI多肽被发现定位于感染细胞的细胞核中。甲型和乙型流感病毒NS1蛋白羧基末端的大量缺失似乎是可以容忍的，而不会影响NS1多肽的功能完整性。
An influenza A virus, A/turkey/Oregon/71, was shown by protein gel analysis to code for an NSi protein approximately half the size of those of other influenza A viruses. Sequence analysis of the NS gene of this virus revealed a 10 nucleotide deletion resulting in an NS1 protein of only 124 amino acids. This truncated NS1 polypeptide retained its karyophilic pattern as detected by indirect immunofluorescence analysis of virus infected cells. Also, A/turkey/Oregon/71 virus grew to high titer in embryonated chicken eggs comparable to other influenza A viruses. We also identified a laboratory variant of an influenza B virus, clone 201, which codes for a truncated NS1 protein. Sequence analysis revealed a 13 nucleotide deletion resulting in a shortened NSi protein of only 127 amino acids as compared to other influenza B virus NS1 proteins possessing a length of 281 amino acids. Again as shown for the NS1 proteins of other influenza B viruses the NSi polypeptide of B virus clone 201 was found to localize in the nucleus of infected cells. It appears that large deletions in the carboxyl terminus of the NS1 proteins of influenza A and B viruses can be tolerated without affecting the functional integrity of the NS1 polypeptide.