Human cerebrospinal fluid regulates proliferation and migration of stem cells through insulin-like growth factor-1.
Human cerebrospinal fluid regulates proliferation and migration of stem cells through insulin-like growth factor-1.
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DOI：
10.1089/scd.2014.0076
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发表时间：
2015-01
影响因子：
4
通讯作者：
Mingxi Zhu;Yun Feng;Sean Dangelmajer;H. Guerrero-Cázares;K. Chaichana;Christopher L. Smith;A. Levchenko;T. Lei;A. Quiñones‐Hinojosa
中科院分区：
文献类型：
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作者：
Mingxi Zhu;Yun Feng;Sean Dangelmajer;H. Guerrero-Cázares;K. Chaichana;Christopher L. Smith;A. Levchenko;T. Lei;A. Quiñones‐Hinojosa
间充质干细胞（MSC）和神经祖细胞（NPC）已被认为是其对中枢神经系统（CNS）病理的临床治疗潜力。它们的潜在效用是其固有的修复受损组织、递送治疗性蛋白质和迁移到脑内病理部位的能力的结果。然而，目前尚不清楚中枢神经系统是否会促进这些潜在治疗细胞的任何变化，这对于在临床应用之前了解这一点至关重要。CNS的主要成分是脑脊液（CSF）。因此，本研究的目的是评估人CSF对人脂肪源性MSC（hAMSC）和人胎儿源性NPC（hfNPC）在细胞增殖、存活和迁移方面的功能的影响。这项研究表明，人非癌CSF促进hAMSC和hfNPC的增殖并抑制其凋亡。在人CSF中预培养这些干细胞也增加了它们的迁移速度和移动距离。此外，人CSF中的胰岛素样生长因子-1（IGF-1）增强了两种干细胞类型中的迁移能力，并增加了C-X-C趋化因子受体4型（CXCR 4）的表达。这些目前的发现强调了一种简单而自然的方式，其中人CSF可以增强人外源性原代hAMSC和hfNPC的增殖、迁移和活力。这项研究可能为改善干细胞治疗CNS病理的临床疗效提供见解。
Mesenchymal stem cells (MSCs) and neural progenitor cells (NPCs) have been regarded for their clinical therapeutic potential for central nervous system (CNS) pathologies. Their potential utility is a result of their intrinsic ability to repair damaged tissues, deliver therapeutic proteins, and migrate to sites of pathology within the brain. However, it remains unclear whether the CNS promotes any changes in these potential therapeutic cells, which would be critical to understand before clinical application. A major component of the CNS is cerebrospinal fluid (CSF). Therefore, the aim of this study was to evaluate the influence that human CSF has on the function of human adipose-derived MSCs (hAMSCs) and human fetal-derived NPCs (hfNPCs) in regard to cell proliferation, survival, and migration. This study demonstrated that human noncancerous CSF promoted proliferation and inhibited apoptosis of hAMSCs and hfNPCs. Preculturing these stem cells in human CSF also increased their migratory speed and distance traveled. Furthermore, insulin-like growth factor-1 (IGF-1) in human CSF enhanced the migration capacity and increased the expression of C-X-C chemokine receptor type 4 (CXCR4) in both stem cell types. These current findings highlight a simple and natural way in which human CSF can enhance the proliferation, migration, and viability of human exogenous primary hAMSCs and hfNPCs. This study may provide insight into improving the clinical efficacy of stem cells for the treatment of CNS pathologies.