In vivo visualization of transplanted pancreatic islets by MRI: comparison between in vivo, histological and electron microscopy findings
In vivo visualization of transplanted pancreatic islets by MRI: comparison between in vivo, histological and electron microscopy findings
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DOI：
10.1002/cmmi.274
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发表时间：
2009-05-01
影响因子：
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通讯作者：
Sbarbati, Andrea
中科院分区：
文献类型：
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作者：
Marzola, Pasquina;Longoni, Biancamaria;Sbarbati, Andrea
这项工作的目的是比较体内MRI可视化的胰岛标记临床级超顺磁性氧化铁（SPIOs）造影剂与离体检查的肝组织在大鼠边缘质量移植的实验模型。将来自Wistar大鼠的700个IEq（胰岛当量）通过门静脉移植到Sprague-Dawley大鼠中，所述IEq通过与Endorem（R）或Resovist（R）温育来标记。在移植后3-42天采集受体大鼠肝脏MR图像。在此期间处死动物，切除其肝脏并制备用于组织学和电子显微镜检查。在MR图像中观察到源自铁颗粒的低信号斑点。对单独斑点的数量进行计数。移植后三天，每三个或四个移植的胰岛观察到一个斑点。移植后7天，组织切片显示胰岛内存在铁。MR图像的时间过程显示，在42天时，Resovist（R）和Endorem（R）的斑点数量分别减少到初始值的65%和22%，而在组织切片中未检测到免疫阳性内分泌细胞。目前的工作表明，胰岛可以标记使用临床批准的SPIO，造影剂和可视化使用体内MRI具有高灵敏度，与文献中的结果一致。与文献中的报告相一致，我们的研究结果表明，铁颗粒可以在肝脏中持续很长一段时间，独立于完整胰岛的存在。版权所有（C）2009约翰威利父子有限公司
The aim of the work was to compare in vivo MRI visualization of pancreatic islets labeled with clinical-grade superparamagnetic iron oxide (SPIOs) contrast agents with ex vivo examination of liver tissue in an experimental model of marginal mass transplantation in rats. Seven hundred IEq (islet Equivalent) from Wistar rats, labeled by incubation with Endorem (R) or Resovist (R), were transplanted into Sprague-Dawley rats through the portal vein. Liver MR images of recipient rats were acquired at different time points (3-42 days) after transplantation. Animals were sacrificed during this period and their livers were excised and prepared for histology and electron microscopy. Hypointense spots originating from iron particles were observed in MR images. The number of separate spots was counted. Three days after transplantation one spot for every three or four transplanted islets was observed. Seven days after transplantation, histological sections showed the presence of iron within pancreatic islets. The time course of MR images showed a decrease in the number of spots, at 42 days, amounting to 65 and 22% of the initial value, for Resovist (R) and Endorem (R) respectively, while no immunopositive endocrine cells were detected in histological slices. The present work shows that pancreatic islets can be labeled using clinically approved SPIO, contrast agents and visualized using in vivo MRI with high sensitivity, consistently with findings in the literature. Differently from reports in the literature, our findings indicate that iron particles could last in the liver for long periods, independently of the presence of intact pancreatic islets. Copyright (C) 2009 John Wiley & Sons, Ltd.