Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique
Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique
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DOI：
10.1088/1758-5090/ab98e5
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发表时间：
2020-10-01
期刊：
影响因子：
9
通讯作者：
Boccaccini, Aldo R.
中科院分区：
文献类型：
--
作者：
Hazur, Jonas;Detsch, Rainer;Boccaccini, Aldo R.
在组织工程领域工作的研究人员已经开发了许多不同的生物织物方法以及各种生物墨水。生物墨水的主要挑战通常仍然是在打印后难以实现形状保真度。为了克服这个问题，开发了一种普遍适用于所有基于藻酸盐的材料的均相预交联技术。在这项研究中，选定的水凝胶后交联后的杨氏模量，以及藻酸盐的M/G比和分子量方面的化学表征，进行了测定。利用我们的技术，可以显著提高2%（w/v）藻酸盐溶液的可印刷性，而不使用更高的聚合物含量、填料或支撑结构。打印高度约为5 mm的3D多孔支架。并研究了不同预交联度的流变行为。对细胞上的剪切力以及在该过程期间打印喷嘴内的生物墨水的流动轮廓进行了估计。可以观察到包埋在新型生物墨水中的打印的NIH/3 T3细胞的高细胞活力在两周的时间段内超过85%。
Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young's Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85% over a time period of two weeks could be observed.