Functional conservations of the alkaline nuclease of herpes simplex type 1 and human cytomegalovirus
Functional conservations of the alkaline nuclease of herpes simplex type 1 and human cytomegalovirus
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DOI：
10.1006/viro.1998.9344
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发表时间：
1998-09-30
期刊：
影响因子：
3.7
通讯作者：
Weinheimer, SP
中科院分区：
文献类型：
--
作者：
Gao, M;Robertson, BJ;Weinheimer, SP
单纯疱疹病毒 1 型 UL12 基因产物碱性核酸酶 (AN) 似乎参与病毒 DNA 加工和衣壳从细胞核中排出（Shao, L., Rapp, L. M. 和 Weller, S. K., Virology 196, 146-162, 1993）。尽管HSV-1 AN对于组织培养中的病毒复制并不是绝对必需的，但AN基因在所有疱疹病毒中的保守性表明AN基因在疱疹病毒的生命周期中发挥着重要作用。人巨细胞病毒 (HCMV) 的 HSV-1 AN 的对应物是 UL98 基因产物。为了检查 HCMV AN 是否可以替代 HSV-1 AN，我们使用携带 HCMV UL98 基因的 HSV-1 扩增子质粒进行了反式互补实验。我们的结果表明 (i) HCMV AN 可以反式补充 HSV-1 AN 缺失突变体 UL12lacZ 病毒的生长； (ii) 一种新的重组病毒，UL12laZcUL98/99，似乎是通过将 HCMV UL98 基因整合到 HSV-1 UL12lacZ 病毒基因组中而产生的； (iii)与其亲本HSV-1 UL12lacZ病毒相比。 UL 12lacZUL98/99 感染的 Vero 细胞中形成的衣壳能够从细胞核转运至细胞质并成熟为感染性病毒。我们的结果表明 AN 在 HSV-1 和 HCMV 之间具有功能保守性。 (C) 1998 年学术出版社。
The herpes simplex virus type 1 UL12 gene product, alkaline nuclease (AN), appears to be involved in viral DNA processing and capsid egress from the nucleus (Shao, L., Rapp, L. M., and Weller, S. K., Virology 196, 146-162, 1993). Although the HSV-1 AN is not absolutely essential for viral replication in tissue culture, conservation of the AN gene in all herpesviruses suggests an important role in the life cycle of herpesviruses. The counterpart of HSV-1 AN for human cytomegalovirus (HCMV) is the UL98 gene product. To examine whether the HCMV AN could substitute for HSV-1 AN, we performed trans-complementation experiments using a HSV-1 amplicon plasmid carrying the HCMV UL98 gene. Our results indicate (i) HCMV AN can complement the growth of the HSV-1 AN deletion mutant UL12lacZ virus in trans; (ii) a new recombinant virus, UL12laZcUL98/99, appears to be generated by the integration of the HCMV UL98 gene into the HSV-1 UL12lacZ viral genome; (iii) in contrast to its parental HSV-1 UL12lacZ virus. capsids formed in UL 12lacZUL98/99-infected Vero cells were able to transport from the nucleus to the cytoplasm and mature into infectious viruses. Our results demonstrate a functional conservation of AN between HSV-1 and HCMV. (C) 1998 Academic Press.