Cholinergic synaptic input to different parts of spiny striatonigral neurons in the rat
Cholinergic synaptic input to different parts of spiny striatonigral neurons in the rat
复制标题
DOI：
10.1002/cne.902690207
复制
发表时间：
1988-03
影响因子：
2.5
通讯作者：
P. Izzo;J. Bolam
中科院分区：
文献类型：
--
作者：
P. Izzo;J. Bolam
通过在电子显微镜下检查对胆碱乙酰转移酶（乙酰胆碱的合成酶）具有免疫反应性的胆碱能纽扣，评估大鼠新纹状体中胆碱能纽扣的突触后目标。这些纽扣与新纹状体神经元形成对称的突触特化。在新纹状体随机搜索中观察到的 209 个免疫反应性突触按钮中，45% 与树突轴接触，34% 与树突棘接触，20% 与神经元周核接触。许多突触后结构具有最常见类型纹状体神经元（中等大小的密集刺神经元）的超微结构特征。通过电子显微镜对高尔基体浸渍的中等大小的多刺神经元切片的检查证实了这一点，这些神经元的切片也经过了胆碱乙酰转移酶的免疫染色。免疫反应性布顿与所检查的神经元的所有部分形成对称的突触特化，即核周、近端和远端树突轴以及树突棘。两个接受胆碱能开关输入的高尔基体中等大小的多刺神经元也被注射到黑质的辣根过氧化物酶逆行标记，因此它们被进一步定性为纹状体黑质神经元。类似地，发现纹状体黑质神经元的七个逆行标记的核周神经元接收来自胆碱能按钮的输入。
The postsynaptic targets of cholinergic boutons in the rat neostriatum were assessed by examination in the electron microscope of boutons that were immunoreactive for choline acetyltransferase, the synthetic enzyme for acetylcholine. These boutons formed symmetrical synaptic specializations with neostriatal neurons. Of 209 immunoreactive synaptic boutons observed in random searches of the neostriatum, 45% made contact with dendritic shafts, 34% with dendritic spines, and 20% with neuronal perikarya. Many of the postsynaptic structures had ultrastructural characteristics of the most common type of striatal neuron, the medium‐size densely spiny neuron. This was confirmed by the examination in the electron microscope of Golgi‐impregnated medium‐size spiny neurons from sections that had also been immunostained for choline acetyltransferase. Immunoreactive boutons formed symmetrical synaptic specializations with all parts of the neurons examined, i.e., perikarya, proximal and distal dendritic shafts, and dendritic spines. Two of the Golgi‐impregnated medium‐size spiny neurons that received input from the cholinergic boutons were also retrogradely labelled with horseradish peroxidase that had been injected into the substantia nigra, they were thus further characterized as striatonigral neurons. Similarly, seven retrogradely labelled perikarya of striatonigral neurons were found to receive input from the cholinergic boutons.