LOW-MASS IONS PRODUCED FROM PEPTIDES BY HIGH-ENERGY COLLISION-INDUCED DISSOCIATION IN TANDEM MASS-SPECTROMETRY
LOW-MASS IONS PRODUCED FROM PEPTIDES BY HIGH-ENERGY COLLISION-INDUCED DISSOCIATION IN TANDEM MASS-SPECTROMETRY
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DOI：
10.1016/1044-0305(93)87006-x
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发表时间：
1993-11-01
影响因子：
3.2
通讯作者：
GIBSON, BW
中科院分区：
文献类型：
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作者：
FALICK, AM;HINES, WM;GIBSON, BW
高能碰撞诱导解离（CID）质谱法为测定多肽的一级序列提供了一种快速、灵敏的方法。分析了大量肽串联CID谱的低质量区（质量低于300）。该质量区包含几种类型的信息片段离子，包括二肽离子、铵离子和其他相关离子。有用的低质量离子也存在于负离子CID光谱中。铵离子（一般结构[H2N=CH-R]+，其中R为氨基酸侧链）和特定氨基酸残基的相关离子特征提供了被分析肽中这些残基存在与否的信息。给出了20种标准氨基酸和一些修饰氨基酸的观察到的铵和相关离子的表。建立了228个多肽高能CID光谱数据库，确定了指示特定氨基酸残基的各种离子的出现频率。两个模型计算机辅助方案分析的氨基酸含量的未知肽已经开发和测试了数据库。
High-energy collision-induced dissociation (CID) mass spectrometry provides a rapid and sensitive means for determining the primary sequence of peptides. The low-mass region (below mass 300) of a large number of tandem CID spectra of peptides has been analyzed. This mass region contains several types of informative fragment ions, including dipeptide ions, immonium ions, and other related ions. Useful low-mass ions are also present in negative-ion CID spectra. Immonium ions (general structure [H2N=CH-R]+, where R is the amino acid side chain) and related ions characteristic of specific amino acid residues give information as to the presence or absence of these residues in the peptide being analyzed. Tables of observed immonium and related ions for the 20 standard amino acids and for a number of modified amino acids are presented. A database consisting of 228 high-energy CID spectra of peptides has been established, and the frequency of occurrence of various ions indicative of specific amino acid residues has been determined. Two model computer-aided schemes for analysis of the amino-acid content of unknown peptides have been developed and tested against the database.