Post-natal development of the porcine microbiota composition and activities
Post-natal development of the porcine microbiota composition and activities
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DOI：
10.1111/j.1462-2920.2006.01009.x
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发表时间：
2006-07-01
影响因子：
5.1
通讯作者：
De Vos, Willem M.
中科院分区：
文献类型：
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作者：
Konstantinov, Sergey R.;Awati, Ajay A.;De Vos, Willem M.
本研究描述了新生期和断奶期猪微生物群的发育及其代谢活动。使用基于16 S rRNA的方法，我们首先分析了出生后第2、5和12天新生仔猪的回肠和结肠微生物群。为了进一步研究3周龄断奶的影响，将19日龄仔猪（n = 64）随机分为两组。在整个研究期间，一半仔猪与母猪在一起，而其余仔猪断奶。16 S rRNA基因扩增子序列分析显示，2日龄仔猪样品中含有大肠杆菌、福氏志贺菌、温和乳杆菌、罗伊氏乳杆菌和嗜酸乳杆菌相关的细菌群。此外，种特异性实时聚合酶链反应检测揭示，L。sobrius和L.罗伊氏菌在新生仔猪和未断奶仔猪的回肠样品中占优势，群体水平高达7 × 108个细胞/克管腔内容物。然而，断奶后，在回肠样品中检测到这两种乳酸杆菌的水平明显较低（< 10（3））。此外，主要微生物群的组成和代谢活动的变化，以及梭菌和E。大肠杆菌，在断奶后早期仔猪的肠道样品中遇到。
The current study describes the development of the porcine microbiota and its metabolic activities during the neonatal and weaning period. Using 16S rRNA-based approaches, we first analysed the ileal and colonic microbiota of neonatal piglets at days 2, 5 and 12 after birth. To further investigate the effect of weaning at 3 weeks of age, 19-day-old piglets (n = 64) were randomly allocated into two groups. Half of the piglets remained with their sows throughout the study, while the remaining piglets were weaned. As revealed by sequence analysis of 16S rRNA gene amplicons, the samples of 2-day-old piglets harboured a consortium of bacteria related to Escherichia coli, Shigella flexneri, Lactobacillus sobrius, Lactobacillus reuteri and Lactobacillus acidophilus. Moreover, species-specific real-time polymerase chain reaction assays unveiled that L. sobrius and L. reuteri predominated in the ileal samples of the neonatal and unweaned piglets with population levels up to 7 x 10(8) cells per gram of lumen content. Following weaning, however, these two lactobacilli were detected at significantly lower levels (< 10(3)) in the ileal samples. Furthermore, a shift in composition and metabolic activities of the predominant microbiota, and emergence of clostridia and E. coli, were encountered in the intestinal samples of the piglets after the early post-weaning period.