Changes in lymphocyte subsets during pregnancy and post-partum in cases of beginning eclampsia
Changes in lymphocyte subsets during pregnancy and post-partum in cases of beginning eclampsia
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DOI：
10.1515/jpm.2000.050
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发表时间：
2000-01-01
影响因子：
2.4
通讯作者：
Schmidt, S
中科院分区：
文献类型：
--
作者：
Kühnert, M;Schmidt, S
目的：本回顾性研究的目的是检测外周血淋巴细胞的表型和激活标志物的表达与妊娠期高血压的发展。方法：16例在妊娠晚期（25-34周）出现高血压的妇女（年龄25-43岁，平均35.1岁）分别在妊娠早期（< 14周）、妊娠晚期（14-23周）、妊娠晚期（36-终止妊娠）和产后1周内采集血样，对照组为16例年龄相匹配的健康孕妇，采用相同的治疗方案。所有血样于早上采集，室温保存，6小时内染色，24小时内测量。根据Dunn的说法，两组间的Kruskal-Wallis方差分析采用多重比较。结果：两组比较，妊娠期及产后总白细胞计数均显著增高。妊娠期高血压患者的CD8+和cd56 +活化T细胞数量在妊娠早期（< 14周）显著升高[p < 0.05]，随后降至正常值。妊娠中期（14-23周）子痫前期辅助/诱导淋巴细胞和cd56 +/ cd3 +淋巴细胞减少，细胞毒性淋巴细胞升高[p]
Aims: The goal of the present retrospective study was to examine the peripheral blood lymphocytes for expression of phenotypic and activation markers concerning the development of hypertension in pregnancy.Methods: 16 women (aged 25-43 years; mean 35.1) developing hypertension in the third trimester (week 25-34) have had blood samples taken in the first(< 14 weeks), the second (week 14-23), the third trimester (week 24-35), in late pregnancy (week 36-termination of pregnancy) and within 1 week post-partum, The control group consisted of 16 age-matched pregnant healthy women, who underwent the same regime. All blood samples were taken in the morning, stored at room temperature and stained within 6 hours and measured within 24 hours. Kruskal-Wallis analysis of variance between both groups was done with multiple comparison according to Dunn.Results: Comparing both groups, the total white cell count was significantly increased in all pregnancies and post-partum. In case of hypertension in pregnancy the cell numbers of suppressor/cytotoxic (CD8+) and CD 56+-activated T cells showed a significant increase in the first trimester (< 14 weeks) [p < 0.05] and decreased thereafter to normal values. In the second trimester (week 14-23) helper/inducer lymphocytes and CD 56+/CD 3+ lymphocytes decreased in case of pre-ecclampsia and cytotoxic lymphocytes elevated [p