Identification and functional characterization of solute carrier family 6 genes in Ciona savignyi
Identification and functional characterization of solute carrier family 6 genes in Ciona savignyi
复制标题
玻璃海鞘溶质载体家族6基因的鉴定及功能表征
DOI：
10.1016/j.gene.2019.04.056
复制
发表时间：
2019-07-15
期刊：
影响因子：
3.5
通讯作者：
Dong，Bo
中科院分区：
文献类型：
--
作者：
Ren，Ping;Wei，Jiankai;Dong，Bo
溶质载体6（solute carrier 6，SLC 6）基因家族作为神经递质转运体，在神经传递、细胞和生物体内平衡中起着重要作用。在本研究中，我们发现SLC6家族基因在脊索动物Cionasavignyi的基因组中有一个扩展。共鉴定出40个候选基因，其中29个完整基因和11个推测基因为SLC6家族基因同源物。系统进化分析表明，CionaSLC6基因与脊椎动物的SLC6基因大多高度保守，但也存在基因重复和基因丢失现象。从SLC6亚家族中选择了4个基因，通过在培养的细胞系中过表达的方法进一步研究它们对细胞生长和迁移的功能特性。结果显示，氨基酸转运蛋白AA1和AA2亚家族的SLC6A7和SLC6A17均显著抑制细胞增殖和迁移。SLC6A1和SLC6A4分别属于GABA和单胺转运蛋白，对HEK293T、HeLa和MCF7细胞的增殖和迁移无影响。整个C.本研究中鉴定的savignyiSLC 6基因为未来SLC 6基因家族的生化、生理和系统发育研究提供了重要的基因组资源。我们的实验数据表明，Ciona氨基酸转运蛋白，如SLC6A7和SLC6A17，是细胞生理和行为所必需的，表明它们在控制海鞘胚胎发生过程中的细胞增殖和迁移中具有关键的潜在作用。
The solute carrier 6 (SLC6) gene family, functioning as neurotransmitter transporters, plays the crucial roles in neurotransmission, cellular and organismal homeostasis. In this study, we found an expansion of SLC6 family gene in the genome of chordate invertebrate Cionasavignyi. A total of 40 candidate genes including 29 complete and 11 putative genes were identified as SLC6 family gene homologs. Phylogenetic analysis revealed that most of theseCionaSLC6 genes were highly conserved with the vertebrate ones, although gene duplication and gene losses did exist. Four genes were selected from SLC6 subfamilies to be further investigated for their functional characteristics on cell growth and migration through overexpression approach in cultured cell lines. The results showed both SLC6A7 and SLC6A17 from amino acid transporters AA1 and AA2 sub-families, respectively, significantly suppressed the cell proliferation and migration. While SLC6A1 and SLC6A4, which were classified into GABA and monoamine transporters, respectively, did not affect the cell proliferation and migration in HEK293T, HeLa, and MCF7 cells. The whole set ofC. savignyiSLC6 genes identified in this study provides an important genomic resource for future biochemical, physiological, and phylogenetic studies on SLC6 gene family. Our experimental data demonstrated thatCionaamino acid transporters, such as SLC6A7 and SLC6A17, were essential for cell physiology and behaviors, indicating their crucially potential roles in the control of cell proliferation and migration during ascidian embryogenesis.