Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.
Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.
复制标题
编码蛋白激酶 C 的 cDNA 克隆的分离：蛋白激酶 C 相关基因家族的证据。
DOI：
--
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
I. Weinstein
中科院分区：
文献类型：
--
作者：
G. Housey;C. O'brian;M. Johnson;P. Kirschmeier;I. Weinstein
我们已经分离出编码蛋白激酶C的cDNA克隆，通过使用53个碱基对的合成寡核苷酸探针，对应于我们从大鼠脑酶获得的肽。我们还使用相同的寡核苷酸探针分离了几个密切相关的克隆。蛋白激酶C克隆之一RP 41的核苷酸序列分析鉴定了224个氨基酸的羧基末端区域，其与cAMP依赖性和cGMP依赖性蛋白激酶的羧基末端催化结构域具有约等于40%的同源性。与RP 41同源的mRNA水平在脑中非常高，而在心脏和肝脏中存在低得多的水平。第二个cDNA克隆RP 16的核苷酸序列分析鉴定了与蛋白激酶C克隆RP 41的相应区域具有65%同源性的推导的氨基酸序列。RP 16对应的mRNA水平在大鼠脑中也很高，但转录本大小和组织特异性表达模式与RP 41不同。这些和额外的结果提供了证据，编码蛋白激酶C的基因是一个新的丝氨酸/苏氨酸蛋白激酶多基因家族的成员。
We have isolated cDNA clones encoding protein kinase C by using a 53-base-pair synthetic oligonucleotide probe corresponding to a peptide that we obtained from the rat brain enzyme. We also have isolated several closely related clones using the same oligonucleotide probe. Nucleotide sequence analysis of one of the protein kinase C clones, RP41, identifies a 224-amino-acid carboxyl-terminal region with approximately equal to 40% homology to the carboxyl-terminal catalytic domains of both the cAMP-dependent and cGMP-dependent protein kinases. The levels of mRNA homologous to RP41 are very high in brain, whereas much lower levels are present in heart and liver. Nucleotide sequence analysis of a second cDNA clone, RP16, identifies a deduced amino acid sequence that shares 65% homology with the corresponding region of the protein kinase C clone RP41. The levels of mRNA corresponding to RP16 are also high in rat brain, but the transcript sizes and tissue-specific expression patterns differ from those of RP41. These and additional results provide evidence that the gene encoding protein kinase C is a member of a novel serine/threonine protein kinase multigene family.