CLONING AND FUNCTIONAL-ANALYSIS OF TIPE, A NOVEL MEMBRANE-PROTEIN THAT ENHANCES DROSOPHILA PARA SODIUM-CHANNEL FUNCTION
CLONING AND FUNCTIONAL-ANALYSIS OF TIPE, A NOVEL MEMBRANE-PROTEIN THAT ENHANCES DROSOPHILA PARA SODIUM-CHANNEL FUNCTION
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DOI：
10.1016/0092-8674(95)90279-1
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发表时间：
1995-09-22
期刊：
影响因子：
64.5
通讯作者：
HALL, LM
中科院分区：
文献类型：
--
作者：
FENG, GP;DEAK, P;HALL, LM
电压依赖性钠通道参与许多可兴奋细胞中动作电位的起始和传播。在这里，我们报告，tipE，一个基因定义的温度敏感性麻痹突变的果蝇，编码一种新的完整的膜蛋白，显着刺激功能表达在非洲爪蟾卵母细胞的果蝇钠通道亚基编码的麻痹（帕拉）轨迹。利用热休克启动子控制转基因果蝇中的tipE（+）基因表达，我们证明了在蛹发育过程中tipE（+）基因表达是拯救成年瘫痪所必需的。此外，我们证明了tipE基因产物在成人中的作用。
Voltage-dependent sodium channels are involved in the initiation and propagation of action potentials in many excitable cells. Here we report that tipE, a gene defined by a temperature-sensitive paralytic mutation in Drosophila, encodes a novel integral membrane protein that dramatically stimulates functional expression in Xenopus oocytes of the Drosophila sodium channel a subunit encoded by the paralytic (para) locus. Using a heat shock promoter to control tipE(+) gene expression in transgenic flies, we demonstrate that tipE(+) gene expression is required during pupal development to rescue adult paralysis. In addition, we demonstrate a role for the tipE gene product in adults.