A whole-mount cholinesterase technique for demonstrating corneal nerves: observations in the albino rabbit.
A whole-mount cholinesterase technique for demonstrating corneal nerves: observations in the albino rabbit.
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用于展示角膜神经的整体胆碱酯酶技术：在白化兔中的观察。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
R. Winkelmann
中科院分区：
文献类型：
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作者：
D. Robertson;R. Winkelmann
采用胆碱酯酶组织化学染色技术对兔全层角膜神经进行了研究。该技术相对简单，产生可重复的结果，可以很好地定义神经纤维模式，并为研究者提供了使用双眼显微镜进行三维研究的优势。当对组织进行特定胆碱酯酶染色时，清晰度足以显示单个轴突及其末端，以及大的神经纤维束。非特异性胆碱酯酶染色显示大束神经纤维，但未显示单个轴突。解剖和胆碱酯酶含量的角膜神经支配的讨论有关的酶证明。
A histochemical staining technique for cholinesterase is reported for the study of corneal nerves in whole-thickness rabbit cornea. The technique is relatively simple, yields reproducible results, allows good definition of the nerve fiber pattern, and provides the investigator, using a binocular microscope, with the advantage of three-dimensional study. When tissue is stained for specific cholinesterase, definition is sufficient to allow visualization of individual axons and their terminations, as well as large nerve fiber bundles. The stain for nonspecific cholinesterase appears to demomtrate the large nerve fiber bundles but not individual axons. The anatomy and cholinesterase content of corneal innervation is discussed in relation to the enzyme demonstrated.