miR-17-5p targets the p300/CBP-associated factor and modulates androgen receptor transcriptional activity in cultured prostate cancer cells.
miR-17-5p targets the p300/CBP-associated factor and modulates androgen receptor transcriptional activity in cultured prostate cancer cells.
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DOI：
10.1186/1471-2407-12-492
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发表时间：
2012-10-24
期刊：
影响因子：
3.8
通讯作者：
Chen XM
中科院分区：
文献类型：
--
作者：
Gong AY;Eischeid AN;Xiao J;Zhao J;Chen D;Wang ZY;Young CY;Chen XM
雄激素受体（AR）信号传导对前列腺癌（PCa）的发生和发展至关重要。AR的转录活性涉及染色质募集共激活因子，包括p300/ cbp相关因子（PCAF）。在前列腺癌的发生和发展过程中，已经发现了不同的miRNA表达谱。miRNAs是否通过调控PCa细胞中PCAF的表达来调控AR的转录活性尚不清楚。采用qRT-PCR、Western blot和免疫细胞化学方法研究了PCAF在多种PCa细胞系中的表达。通过染色质免疫沉淀、报告基因构建分析和MTS法检测PCAF表达对ar调控的PCa细胞转录活性和细胞生长的影响。使用荧光素酶报告基因法评估miR-17-5p对PCAF的靶向作用。PCAF在多种PCa细胞系中表达上调。在培养的PCa细胞中，PCAF的上调促进AR转录激活和细胞生长。PCAF在PCa细胞中的表达与miR-17-5p的下调有关。miR-17-5p靶向PCAF mRNA的3 ' -非翻译区，导致翻译抑制和RNA降解，从而调节PCa细胞中AR的转录活性。PCAF在培养的PCa细胞中上调，PCAF的上调与miR-17-5p的下调相关。在培养的PCa细胞中，miR-17-5p靶向PCAF可调节AR转录活性和细胞生长。
Androgen receptor (AR) signalling is critical to the initiation and progression of prostate cancer (PCa). Transcriptional activity of AR involves chromatin recruitment of co-activators, including the p300/CBP-associated factor (PCAF). Distinct miRNA expression profiles have been identified in PCa cells during the development and progression of the disease. Whether miRNAs regulate PCAF expression in PCa cells to regulate AR transcriptional activity is still unclear. Expression of PCAF was investigated in several PCa cell lines by qRT-PCR, Western blot, and immunocytochemistry. The effects of PCAF expression on AR-regulated transcriptional activity and cell growth in PCa cells were determined by chromatin immunoprecipitation, reporter gene construct analysis, and MTS assay. Targeting of PCAF by miR-17-5p was evaluated using the luciferase reporter assay. PCAF was upregulated in several PCa cell lines. Upregulation of PCAF promoted AR transcriptional activation and cell growth in cultured PCa cells. Expression of PCAF in PCa cells was associated with the downregulation of miR-17-5p. Targeting of the 3’-untranslated region of PCAF mRNA by miR-17-5p caused translational suppression and RNA degradation, and, consequently, modulation of AR transcriptional activity in PCa cells. PCAF is upregulated in cultured PCa cells, and upregulation of PCAF is associated with the downregulation of miR-17-5p. Targeting of PCAF by miR-17-5p modulates AR transcriptional activity and cell growth in cultured PCa cells.
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影响因子：
56.9
作者：
Triboulet, Robinson;Mari, Bernard;Benkirane, Monsef
通讯作者：
Benkirane, Monsef
DOI：
10.1086/648589
发表时间：
2010-01-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Gong AY;Zhou R;Hu G;Liu J;Sosnowska D;Drescher KM;Dong H;Chen XM
通讯作者：
Chen XM
影响因子：
14.9
作者：
Gaughan L;Logan IR;Neal DE;Robson CN
通讯作者：
Robson CN
影响因子：
4
作者：
Culig, Zoran;Bartsch, Georg
通讯作者：
Bartsch, Georg
影响因子：
--
作者：
Reutens, AT;Fu, MF;Pestell, RG
通讯作者：
Pestell, RG